Publication result detail
Magnetic Microspheres for DNA Diagnostics and DNA Delivery
RITTICH, B.; ŠPANOVÁ, A.; TRACHTOVÁ, Š.; HORÁK, D.
Original Title
Magnetic Microspheres for DNA Diagnostics and DNA Delivery
English Title
Magnetic Microspheres for DNA Diagnostics and DNA Delivery
Type
Abstract
Original Abstract
Magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) [P(HEMA-EDMA))], poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [P(HEMA-GMA)], and poly(glycidyl methacrylate) [PGMA] microspheres were prepared by a single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in molecular diagnostic was demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic and iminodiacetic acid immobilised on magnetic PGMA and P(HEMA-EDMA) microspheres, respectively, were used as catalysts of plasmid DNA cleavage. Monodisperse poly(N-isopropylacrylamide) microparticles were prepared by two methods, namely, ammonium persulfate-initiated precipitation polymerization in water and 2,2'-azobisisobutyronitrile-initiated and poly(hydrogenated isoprene-block-styrene)-stabilized dispersion polymerization in toluene/heptane. Microspheres were applied for reversible adsorption of DNA at different temperatures.
English abstract
Magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) [P(HEMA-EDMA))], poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [P(HEMA-GMA)], and poly(glycidyl methacrylate) [PGMA] microspheres were prepared by a single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in molecular diagnostic was demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic and iminodiacetic acid immobilised on magnetic PGMA and P(HEMA-EDMA) microspheres, respectively, were used as catalysts of plasmid DNA cleavage. Monodisperse poly(N-isopropylacrylamide) microparticles were prepared by two methods, namely, ammonium persulfate-initiated precipitation polymerization in water and 2,2'-azobisisobutyronitrile-initiated and poly(hydrogenated isoprene-block-styrene)-stabilized dispersion polymerization in toluene/heptane. Microspheres were applied for reversible adsorption of DNA at different temperatures.
Keywords
nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; poly(N-isopropylacrylamide); enzyme immobilization; DNA isolation; PCR
Key words in English
nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; poly(N-isopropylacrylamide); enzyme immobilization; DNA isolation; PCR
Authors
RITTICH, B.; ŠPANOVÁ, A.; TRACHTOVÁ, Š.; HORÁK, D.
RIV year
2012
Released
30.06.2010
Publisher
VUTIUM
Location
Brno
ISBN
978-80-214-4112-5
Book
Book of abstract 6th International Conference on Materials Structure and Micromechanics of Fracture.
Pages from
151
Pages to
151
Pages count
1
BibTex
@misc{BUT72665,
author="RITTICH, B. and ŠPANOVÁ, A. and TRACHTOVÁ, Š. and HORÁK, D.",
title="Magnetic Microspheres for DNA Diagnostics and DNA Delivery",
booktitle="Book of abstract 6th International Conference on Materials Structure and Micromechanics of Fracture.",
year="2010",
pages="151--151",
publisher="VUTIUM",
address="Brno",
isbn="978-80-214-4112-5",
note="Abstract"
}