Publication detail
Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.
ŠPANOVÁ, A. TRACHTOVÁ, Š. TVRDÍKOVÁ, J. HORÁK, D. RITTICH, B.
Original Title
Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.
Type
abstract
Language
English
Original Abstract
Non-selective isolation of high-quality DNA can be performed using carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. Analogically the magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation as well as lanthanum manganese perovskites nanoparticles encapsulated into a thin silica shell.
Keywords
magnetic hydrophilic microspheres; P(HEMA-co-GMA); perovskites nanoparticles; PCR-ready DNA; PCR
Authors
ŠPANOVÁ, A.; TRACHTOVÁ, Š.; TVRDÍKOVÁ, J.; HORÁK, D.; RITTICH, B.
Released
28. 6. 2010
Publisher
VUTIUM, Brno
Location
Brno
ISBN
978-80-214-4112-5
Book
In Book of Abstract Sixth International Conference on Materials Structure and Micromechanics of Fracture
Pages from
152
Pages to
152
Pages count
1
BibTex
@misc{BUT72667,
author="Alena {Španová} and Štěpánka {Trachtová} and Jana {Tvrdíková} and Daniel {Horák} and Bohuslav {Rittich}",
title="Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.",
booktitle="In Book of Abstract Sixth International Conference on Materials Structure and Micromechanics of Fracture",
year="2010",
pages="152--152",
publisher="VUTIUM, Brno",
address="Brno",
isbn="978-80-214-4112-5",
note="abstract"
}