Detail publikačního výsledku

Utilization of linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process

LAŠTOVIČKOVÁ, M.; MAZANEC, K.; BENKOVSKÁ, D.; BOBÁĽOVÁ, J.

Original Title

Utilization of linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process

English Title

Utilization of linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process

Type

Peer-reviewed article not indexed in WoS or Scopus

Original Abstract

The analysis of intact proteins, extracted from the barley grain, the malt and the aliquots collected during malting and presented in this paper, show the feasibility of using the linear mode of MALDI-TOF MS to obtain a profile of the proteins present at all stages of the malting process. The best and the most reproducible results were obtained after a desalting procedure using Nanosep 3K and applying DHAP as a matrix for the MS measurement of the intact proteins.

English abstract

The analysis of intact proteins, extracted from the barley grain, the malt and the aliquots collected during malting and presented in this paper, show the feasibility of using the linear mode of MALDI-TOF MS to obtain a profile of the proteins present at all stages of the malting process. The best and the most reproducible results were obtained after a desalting procedure using Nanosep 3K and applying DHAP as a matrix for the MS measurement of the intact proteins.

Keywords

barley, brewing, glycation, malt, MALDI-MS, protein

Key words in English

barley, brewing, glycation, malt, MALDI-MS, protein

Authors

LAŠTOVIČKOVÁ, M.; MAZANEC, K.; BENKOVSKÁ, D.; BOBÁĽOVÁ, J.

RIV year

2012

Released

01.11.2010

ISBN

0046-9750

Periodical

JOURNAL OF THE INSTITUTE OF BREWING

Volume

116

Number

3

State

United Kingdom of Great Britain and Northern Ireland

Pages from

245

Pages to

250

Pages count

6

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@article{BUT73604,
  author="Markéta {Laštovičková} and Karel {Mazanec} and Dagmar {Benkovská} and Janette {Bobáľová}",
  title="Utilization of linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process",
  journal="JOURNAL OF THE INSTITUTE OF BREWING",
  year="2010",
  volume="116",
  number="3",
  pages="245--250",
  issn="0046-9750"
}