Publication result detail
Concanavalin A lectin-based affinity chromatography for enrichment and determination of glycoproteins in barley malt
BENKOVSKÁ, D.; FLODROVÁ, D.; BOBÁĽOVÁ, J.
Original Title
Concanavalin A lectin-based affinity chromatography for enrichment and determination of glycoproteins in barley malt
English Title
Concanavalin A lectin-based affinity chromatography for enrichment and determination of glycoproteins in barley malt
Type
Abstract
Original Abstract
In this study, glycoproteins of barley malt were investigated. Glycosylated proteins were first separated from non-glycosylated proteins using a new HPLC monolithic ConA affinity column (Dionex), then separated on SDS-PAGE, digested and identified using MALDI-TOF/TOF mass spectrometer.
English abstract
In this study, glycoproteins of barley malt were investigated. Glycosylated proteins were first separated from non-glycosylated proteins using a new HPLC monolithic ConA affinity column (Dionex), then separated on SDS-PAGE, digested and identified using MALDI-TOF/TOF mass spectrometer.
Keywords
glycoprotein, glycosylation, concanavalin A, barley, malt, MALDI
Key words in English
glycoprotein, glycosylation, concanavalin A, barley, malt, MALDI
Authors
BENKOVSKÁ, D.; FLODROVÁ, D.; BOBÁĽOVÁ, J.
RIV year
2012
Released
14.09.2011
ISBN
0009-2770
Periodical
CHEMICKE LISTY
Volume
105
Number
18
State
Czech Republic
Pages from
s1008
Pages to
s1009
Pages count
2