Publication result detail

PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine

ŠURANSKÁ, H.; VRÁNOVÁ, D.; JIŘÍKOVÁ, I.; PROCHÁZKOVÁ, L.; VADKERTIOVÁ, R.; OMELKOVÁ, J.

Original Title

PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine

English Title

PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine

Type

Abstract

Original Abstract

The aim of this study was to identify yeasts isolated from red wine of Rulandske modre variety (Vitis vinifera) from integrated and ecological agriculture mode (production) from South Moravia. The yeast strains were isolated in regular intervals during spontaneous fermented must (without any SO2 addition). Together, we identified 124 strains (60 from ecological; 64 from integrated fermented must). As a rapid identification method, we used PCR-RFLP (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism) and by application of specific primers (ITS1-ITS4) we amplified 5.8S-ITS segment of rDNA. PCR-RFLP of ITS regions using HaeIII, HinfI, TaqI, AluI and MseI (TruI) was used for identification and grouping of genetically similar strains. Restriction fragments were detected by agarose gel electrophoresis. All obtained electrophoreograms were processed by software BioNumerics 6.5 and UPGMA cluster analysis.

English abstract

The aim of this study was to identify yeasts isolated from red wine of Rulandske modre variety (Vitis vinifera) from integrated and ecological agriculture mode (production) from South Moravia. The yeast strains were isolated in regular intervals during spontaneous fermented must (without any SO2 addition). Together, we identified 124 strains (60 from ecological; 64 from integrated fermented must). As a rapid identification method, we used PCR-RFLP (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism) and by application of specific primers (ITS1-ITS4) we amplified 5.8S-ITS segment of rDNA. PCR-RFLP of ITS regions using HaeIII, HinfI, TaqI, AluI and MseI (TruI) was used for identification and grouping of genetically similar strains. Restriction fragments were detected by agarose gel electrophoresis. All obtained electrophoreograms were processed by software BioNumerics 6.5 and UPGMA cluster analysis.

Keywords

wine, yeasts, PCR RFLP

Key words in English

wine, yeasts, PCR RFLP

Authors

ŠURANSKÁ, H.; VRÁNOVÁ, D.; JIŘÍKOVÁ, I.; PROCHÁZKOVÁ, L.; VADKERTIOVÁ, R.; OMELKOVÁ, J.

RIV year

2012

Released

28.10.2011

Publisher

Masarykova universita

Location

Brno

ISBN

978-80-210-5594-0

Book

XV. Setkání biochemiků a molekulárních biologů: Sborník příspěvků

Pages from

95

Pages to

95

Pages count

1

Full text in the Digital Library

http://hdl.handle.net/

BibTex

@misc{BUT74869,
  author="Hana {Šuranská} and Dana {Vránová} and Ivana {Jiříková} and Lenka {Procházková} and Renata {Vadkertiová} and Jiřina {Omelková}",
  title="PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine",
  booktitle="XV. Setkání biochemiků a molekulárních biologů: Sborník příspěvků",
  year="2011",
  edition="1",
  pages="95--95",
  publisher="Masarykova universita",
  address="Brno",
  isbn="978-80-210-5594-0",
  note="Abstract"
}