R&D result detail

Original Title

Determination of Eight Polycyclic Aromatic Hydrocarbons and in Pea Plants (Pisum sativum L.) Extracts by High Performance Liquid Chromatography with Electrochemical Detection

English Title

Determination of Eight Polycyclic Aromatic Hydrocarbons and in Pea Plants (Pisum sativum L.) Extracts by High Performance Liquid Chromatography with Electrochemical Detection

Type

WoS Article

Original Abstract

In the field of plant physiology, knowledge about polycyclic aromatic hydrocarbons (PAHs), mechanisms of actions are still missing and are limited despite the fact that induction of oxidative stress by several PAHs has been demonstrated. Primarily, high performance liquid chromatography coupled with UV detector and high performance liquid chromatography coupled with electrochemical detector were optimized for detection and quantification of the following PAHs as fluoranthene, pyrene, benzo[a]pyrene, naphthalene, benzo[a]antracene, methylanthracene, triphenylene and coronene. Optimised HPLC method enabled determination of fluoranthene transport into different plant organs - roots, stems and leaves. Hydroponically cultivated pea plants also proved as suitable model for monitoring of effect of fluoranthene on uptake and transport of physiologically crucial elements as calcium, copper, iron and zinc. Significant differences between control and treated plants were determined, however, role of these elements in responses to fluoranthene treatment should be further investigated.

English abstract

In the field of plant physiology, knowledge about polycyclic aromatic hydrocarbons (PAHs), mechanisms of actions are still missing and are limited despite the fact that induction of oxidative stress by several PAHs has been demonstrated. Primarily, high performance liquid chromatography coupled with UV detector and high performance liquid chromatography coupled with electrochemical detector were optimized for detection and quantification of the following PAHs as fluoranthene, pyrene, benzo[a]pyrene, naphthalene, benzo[a]antracene, methylanthracene, triphenylene and coronene. Optimised HPLC method enabled determination of fluoranthene transport into different plant organs - roots, stems and leaves. Hydroponically cultivated pea plants also proved as suitable model for monitoring of effect of fluoranthene on uptake and transport of physiologically crucial elements as calcium, copper, iron and zinc. Significant differences between control and treated plants were determined, however, role of these elements in responses to fluoranthene treatment should be further investigated.

Keywords

Polycyclic Aromatic Hydrocarbons; High Performance Liquid Chromatography with UV and Electrochemical Detection; Fluoranthene; Pea Plants; Pisum sativum

Key words in English

Polycyclic Aromatic Hydrocarbons; High Performance Liquid Chromatography with UV and Electrochemical Detection; Fluoranthene; Pea Plants; Pisum sativum

Authors

ZÍTKA, O.; BABULA, P.; SOCHOR, J.; KUMMEROVÁ, M.; KRYŠTOFOVÁ, O.; ADAM, V.; HAVEL, L.; BEKLOVÁ, M.; HUBÁLEK, J.; KIZEK, R.

RIV year

2013

Released

01.02.2012

ISBN

1452-3981

Periodical

International Journal of Electrochemical Science

Volume

7

Number

2

State

Republic of Serbia

Pages from

908

Pages to

927

Pages count

20

BibTex

@article{BUT92984,
  author="Ondřej {Zítka} and Petr {Babula} and Jiří {Sochor} and Marie {Kummerová} and Olga {Kryštofová} and Vojtěch {Adam} and Ladislav {Havel} and Miroslava {Beklová} and Jaromír {Hubálek} and René {Kizek}",
  title="Determination of Eight Polycyclic Aromatic Hydrocarbons and in Pea Plants (Pisum sativum L.) Extracts by High Performance Liquid Chromatography with Electrochemical Detection",
  journal="International Journal of Electrochemical Science",
  year="2012",
  volume="7",
  number="2",
  pages="908--927",
  issn="1452-3981"
}