Publication result detail
Identification and determination of relatedness of lactobacilli using different DNA amplification methods
TURKOVÁ, K.; RITTICH, B.; ŠPANOVÁ, A.
Original Title
Identification and determination of relatedness of lactobacilli using different DNA amplification methods
English Title
Identification and determination of relatedness of lactobacilli using different DNA amplification methods
Type
Peer-reviewed article not indexed in WoS or Scopus
Original Abstract
Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.
English abstract
Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.
Keywords
Lactobacillus, breastfed infants, dairy products, identification, PCR, fingerprinting
Key words in English
Lactobacillus, breastfed infants, dairy products, identification, PCR, fingerprinting
Authors
TURKOVÁ, K.; RITTICH, B.; ŠPANOVÁ, A.
RIV year
2013
Released
01.09.2012
Publisher
Springer
ISBN
0366-6352
Periodical
Chemical Papers
Volume
66
Number
9
State
Slovak Republic
Pages from
842
Pages to
851
Pages count
10
BibTex
@article{BUT93027,
author="Kristýna {Turková} and Bohuslav {Rittich} and Alena {Španová}",
title="Identification and determination of relatedness of lactobacilli using different DNA amplification methods",
journal="Chemical Papers",
year="2012",
volume="66",
number="9",
pages="842--851",
issn="0366-6352"
}