Detail publikačního výsledku
The Use of Real Time PCR Method for Evaluation of Magnetic Microspheres
TRACHTOVÁ, Š.; RITTICH, B.; HORÁK, D.; ŠPANOVÁ, A.
Original Title
The Use of Real Time PCR Method for Evaluation of Magnetic Microspheres
English Title
The Use of Real Time PCR Method for Evaluation of Magnetic Microspheres
Type
Abstract
Original Abstract
The real-time quantitative PCR (qPCR) was used for the evaluation of non-porous HEMA based magnetic microspheres on the PCR course (PCR sensitivity). It was shown that 1-10 ul microsphere suspensions (2 mg/ml) in PCR mixtures do not interfere in the PCR. It can be assumed that the magnetic nuclei were covered with polymer layer.
English abstract
The real-time quantitative PCR (qPCR) was used for the evaluation of non-porous HEMA based magnetic microspheres on the PCR course (PCR sensitivity). It was shown that 1-10 ul microsphere suspensions (2 mg/ml) in PCR mixtures do not interfere in the PCR. It can be assumed that the magnetic nuclei were covered with polymer layer.
Keywords
magnetic microspheres, real time PCR
Key words in English
magnetic microspheres, real time PCR
Authors
TRACHTOVÁ, Š.; RITTICH, B.; HORÁK, D.; ŠPANOVÁ, A.
RIV year
2013
Released
31.10.2012
Publisher
Institute of Analytical chemstry AS CR
Location
Brno, Czech Republic
ISBN
978-80-904959-1-3
Book
CECE 2012, 9th International Interdisciplinary Meeting on Bioanalysis
Edition
1
Pages from
374
Pages to
378
Pages count
5
Full text in the Digital Library
BibTex
@misc{BUT94933,
author="Štěpánka {Trachtová} and Bohuslav {Rittich} and Daniel {Horák} and Alena {Španová}",
title="The Use of Real Time PCR Method for Evaluation of Magnetic Microspheres",
booktitle="CECE 2012, 9th International Interdisciplinary Meeting on Bioanalysis",
year="2012",
series="1",
edition="1",
pages="374--378",
publisher="Institute of Analytical chemstry AS CR",
address="Brno, Czech Republic",
isbn="978-80-904959-1-3",
note="Abstract"
}