Publication detail

Multiresidue mycotoxin analysis of conventional and organic agriculture commodities by UPLC-MS/MS

BOLECHOVÁ, M. KOSUBOVÁ, P. ČÁSLAVSKÝ, J. POSPÍCHALOVÁ, M.

Original Title

Type

conference paper

Language

English

Original Abstract

Alkaloids are widely distributed plant metabolites, which are grouped by typical structural characteristics into large families such as pyrrolizidine alkaloids (PAs), which include more than 350 individual heterocyclic compounds. Intoxications caused by PAs are described by hepatotoxicity with tendency to cause hemorrhagic necrosis and veno-occlusion in liver, pneumotoxicity, carcinogenity, mutagenity and genotoxicity. Thus there is a need for methods that allow determination of these dangerous plant toxins. In this study fast, reliable and sensitive approach is proposed allowing simultaneous screening, identification, and quantification of PAs in feed samples. PAs including monocrotaline, senkirkine, senecionine, seneciphylline and retrorsine were determined by ultra performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Sample preparation is based on modified QuEChERS approach. Mean recovery, precision, matrix effects and limits of quantification (LOQ) were assessed for four matrices within the method validation. The presented method was tested on 41 various feed samples of Czech production, where presence of pyrrolizidine alkaloids was expected. The most abundant PA was seneciphylline, while monocrotaline, retrorsine, senecionine and senkirkine were found in lower quantities. Feed samples with high content of chlorophyll contained the highest levels of PAs, in general.U legislation related to feeds is focused on production and distribution of feeds that will not cause any risks for humans, animals and/or environment. Directive 2002/32/ES and Recommendation 2006/576/ES indicate guidance values for the presence of some mycotoxins that shall not be exceeded. UKZUZ has been checking raw materials and feed samples from all steps of feedstuffs production since 2004 on a regular basis. Aflatoxins B1, B2, G1, G2, Fumonisins B1, B2, Ochratoxin A, Deoxynivalenol (DON), Zearalenon (ZON) and T-2 and HT-2 toxins were determined in maize, wheat, barley, silage, haylage, grass hay, and complementary and complete feedstuffs. For future needs of official controls a multiresidual method based on modified unbuffered QuEChERS sample preparation and UPLC-MS/MS determination is under development. This method will enable to increase number of samples and also number of mycotoxins present in feeds.

Keywords

mycotoxin, multiresidue method, chromatography, mass spectrometry

Authors

BOLECHOVÁ, M.; KOSUBOVÁ, P.; ČÁSLAVSKÝ, J.; POSPÍCHALOVÁ, M.

Released

21. 1. 2013

Publisher

Bastiaanse Communication

Location

Bilthoven the Netherlands

Pages from

105

Pages to

105

Pages count

BibTex

@inproceedings{BUT97512,
  author="BOLECHOVÁ, M. and KOSUBOVÁ, P. and ČÁSLAVSKÝ, J. and POSPÍCHALOVÁ, M.",
  title="Multiresidue mycotoxin analysis of conventional and organic agriculture commodities by UPLC-MS/MS",
  year="2013",
  pages="105--105",
  publisher="Bastiaanse Communication",
  address="Bilthoven the Netherlands"
}