R&D result detail

Original Title

Antibacterial activity and cell viability of hyaluronan fiber with silver nanoparticles

English Title

Antibacterial activity and cell viability of hyaluronan fiber with silver nanoparticles

Type

Peer-reviewed article not indexed in WoS or Scopus

Original Abstract

Silver has been used since time immemorial in different chemical form to treat burns, wounds and several different infections caused by pathogenic bacteria, advancement of biological process of nanoparticles synthesis is evolving into a key area of nanotechnology. The current study deals with the green synthesis, characterization, and evaluation of the biological activity and cell viability of hyaluronan fibers with incorporated silver nanoparticles (HA-Ag NPs). Hyaluronan fiber was prepared by the dissolving of sodium hyaluronate (HA) in aqueous alkaline solution to prepare a transparent solution, which was used for the preparation of fibers by a wet-spinning technique. Consequently, hyaluronan fiber was used as capping and stabilizing agent for the preparation of fibers with silver nanoparticles. HA-Ag NPs were confirmed by transmission electron microscopy, dynamic light scattering, UV/VIS spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, thermal analysis, nuclear magnetic resonance, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. HA-Ag NPs showed high antibacterial activity of against Staphylococcus aureus and Escherichia coli. Cell viability tests indicated that hyaluronan, hyaluronan fibers and hyaluronan fibers with silver nanoparticles were non-toxic on the cell growth. Two different particles size of Ag NPs (10, 40 nm) had not any toxicity till the concentration limit. These tests were performed using mouse fibroblast cell line 3T3.

English abstract

Silver has been used since time immemorial in different chemical form to treat burns, wounds and several different infections caused by pathogenic bacteria, advancement of biological process of nanoparticles synthesis is evolving into a key area of nanotechnology. The current study deals with the green synthesis, characterization, and evaluation of the biological activity and cell viability of hyaluronan fibers with incorporated silver nanoparticles (HA-Ag NPs). Hyaluronan fiber was prepared by the dissolving of sodium hyaluronate (HA) in aqueous alkaline solution to prepare a transparent solution, which was used for the preparation of fibers by a wet-spinning technique. Consequently, hyaluronan fiber was used as capping and stabilizing agent for the preparation of fibers with silver nanoparticles. HA-Ag NPs were confirmed by transmission electron microscopy, dynamic light scattering, UV/VIS spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, thermal analysis, nuclear magnetic resonance, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. HA-Ag NPs showed high antibacterial activity of against Staphylococcus aureus and Escherichia coli. Cell viability tests indicated that hyaluronan, hyaluronan fibers and hyaluronan fibers with silver nanoparticles were non-toxic on the cell growth. Two different particles size of Ag NPs (10, 40 nm) had not any toxicity till the concentration limit. These tests were performed using mouse fibroblast cell line 3T3.

Keywords

Green chemistry, Hyaluronan fiber, Silver nanoparticles, Cell viability, Antibacterial activity, Medical applications

Key words in English

Green chemistry, Hyaluronan fiber, Silver nanoparticles, Cell viability, Antibacterial activity, Medical applications

Authors

ABDEL-MOHSEN, A.M.; HRDINA, R.; BURGERT, L.; ABDEL-RAHMAN, R. M.; HAŠOVÁ, M.; ŠMEJKALOVÁ, D.; KOLÁŘ, M.; PEKAŘ, M.; ALY, A.S.

RIV year

2013

Released

01.11.2012

ISBN

0144-8617

Periodical

Carbohydrate Polymers

Volume

92

Number

2

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1177

Pages to

1187

Pages count

11

BibTex

@article{BUT98008,
  author="ABDEL-MOHSEN, A.M. and HRDINA, R. and BURGERT, L. and ABDEL-RAHMAN, R. M. and HAŠOVÁ, M. and ŠMEJKALOVÁ, D. and KOLÁŘ, M. and PEKAŘ, M. and ALY, A.S.",
  title="Antibacterial activity and cell viability of hyaluronan fiber with silver nanoparticles",
  journal="Carbohydrate Polymers",
  year="2012",
  volume="92",
  number="2",
  pages="1177--1187",
  issn="0144-8617"
}