Publication result detail
DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices
CHROBOKOVÁ, M.; RITTICH, B.; ŠPANOVÁ, A.; HORÁK, D.
Original Title
DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices
English Title
DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices
Type
Paper in proceedings (conference paper)
Original Abstract
Isolation of DNA is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction (PCR). Total DNA was successfully isolated from cell lysates using magnetic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres covered by carboxyl groups in the presence of PEG 6000 and sodium chloride. DNA isolation by magnetic particles is comparable to the traditional extraction procedures such as phenol/chloroform one and non-magnetic silica particles but with the added advantages of reduced organic solvent consumption and processing time.
English abstract
Isolation of DNA is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction (PCR). Total DNA was successfully isolated from cell lysates using magnetic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres covered by carboxyl groups in the presence of PEG 6000 and sodium chloride. DNA isolation by magnetic particles is comparable to the traditional extraction procedures such as phenol/chloroform one and non-magnetic silica particles but with the added advantages of reduced organic solvent consumption and processing time.
Keywords
magnetic particles, DNA, microorganisms, foods; polymerase chain reaction
Key words in English
magnetic particles, DNA, microorganisms, foods; polymerase chain reaction
Authors
CHROBOKOVÁ, M.; RITTICH, B.; ŠPANOVÁ, A.; HORÁK, D.
RIV year
2016
Released
21.03.2013
Publisher
Československá spoločnosť mikrobiologická
Location
Bratislava-Praha
ISBN
978-80-971422-0-9
Book
Čo nového v mikrobiologii
Pages from
94
Pages to
98
Pages count
222
BibTex
@inproceedings{BUT99830,
author="Maria {Chroboková} and Bohuslav {Rittich} and Alena {Španová} and Daniel {Horák}",
title="DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices",
booktitle="Čo nového v mikrobiologii",
year="2013",
pages="94--98",
publisher="Československá spoločnosť mikrobiologická",
address="Bratislava-Praha",
isbn="978-80-971422-0-9"
}