Publication result detail

Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; KOUŘILOVÁ, X.; KALINA, M.; KOVALČÍK, A.; KOLLER, M.; NEBESAROVA, J.; KRZYZANEK, V.; HRUBANOVA, K.; MÁSILKO, J.; SLANINOVÁ, E.; OBRUČA, S.

Original Title

Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium

English Title

Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium

Type

WoS Article

Original Abstract

Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolateAneurinibacillussp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 degrees C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated thatAneurinibacillussp. H1 is a very promising candidate for production of PHA with tailored material properties.

English abstract

Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolateAneurinibacillussp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 degrees C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated thatAneurinibacillussp. H1 is a very promising candidate for production of PHA with tailored material properties.

Keywords

polyhydroxyalkanoates; thermophiles; Aneurinibacillussp; P(3HB-co-4HB); P(3HB-co-3HV-co-4HB)

Key words in English

polyhydroxyalkanoates; thermophiles; Aneurinibacillussp; P(3HB-co-4HB); P(3HB-co-3HV-co-4HB)

Authors

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; KOUŘILOVÁ, X.; KALINA, M.; KOVALČÍK, A.; KOLLER, M.; NEBESAROVA, J.; KRZYZANEK, V.; HRUBANOVA, K.; MÁSILKO, J.; SLANINOVÁ, E.; OBRUČA, S.

RIV year

2021

Released

29.05.2020

Publisher

MDPI

Location

Switzerland

ISBN

2073-4360

Periodical

Polymers

Volume

12

Number

6

State

Swiss Confederation

Pages from

1

Pages to

13

Pages count

13

URL

https://www.mdpi.com/2073-4360/12/6/1235

Full text in the Digital Library

http://hdl.handle.net/11012/193675

BibTex

@article{BUT164652,
  author="Iva {Buchtíková} and Ivana {Nováčková} and Petr {Sedláček} and Xenie {Kouřilová} and Michal {Kalina} and Adriána {Kovalčík} and Martin {Koller} and Jana {Nebesářová} and Vladislav {Krzyžánek} and Kamila {Hrubanová} and Jiří {Másilko} and Eva {Slaninová} and Stanislav {Obruča}",
  title="Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium",
  journal="Polymers",
  year="2020",
  volume="12",
  number="6",
  pages="1--13",
  doi="10.3390/polym12061235",
  url="https://www.mdpi.com/2073-4360/12/6/1235"
}

Documents

Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers 1 Isolation and Characterization of the Bacterium
polymers-12-01235