Publication result detail
Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium
PERNICOVÁ, I.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; KOUŘILOVÁ, X.; KALINA, M.; KOVALČÍK, A.; KOLLER, M.; NEBESAROVA, J.; KRZYZANEK, V.; HRUBANOVA, K.; MÁSILKO, J.; SLANINOVÁ, E.; OBRUČA, S.
Original Title
Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium
English Title
Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium
Type
WoS Article
Original Abstract
Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolateAneurinibacillussp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 degrees C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated thatAneurinibacillussp. H1 is a very promising candidate for production of PHA with tailored material properties.
English abstract
Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolateAneurinibacillussp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 degrees C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated thatAneurinibacillussp. H1 is a very promising candidate for production of PHA with tailored material properties.
Keywords
polyhydroxyalkanoates; thermophiles; Aneurinibacillussp; P(3HB-co-4HB); P(3HB-co-3HV-co-4HB)
Key words in English
polyhydroxyalkanoates; thermophiles; Aneurinibacillussp; P(3HB-co-4HB); P(3HB-co-3HV-co-4HB)
Authors
PERNICOVÁ, I.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; KOUŘILOVÁ, X.; KALINA, M.; KOVALČÍK, A.; KOLLER, M.; NEBESAROVA, J.; KRZYZANEK, V.; HRUBANOVA, K.; MÁSILKO, J.; SLANINOVÁ, E.; OBRUČA, S.
RIV year
2021
Released
29.05.2020
Publisher
MDPI
Location
Switzerland
ISBN
2073-4360
Periodical
Polymers
Volume
12
Number
6
State
Swiss Confederation
Pages from
1
Pages to
13
Pages count
13
URL
Full text in the Digital Library
BibTex
@article{BUT164652,
author="Iva {Buchtíková} and Ivana {Nováčková} and Petr {Sedláček} and Xenie {Kouřilová} and Michal {Kalina} and Adriána {Kovalčík} and Martin {Koller} and Jana {Nebesářová} and Vladislav {Krzyžánek} and Kamila {Hrubanová} and Jiří {Másilko} and Eva {Slaninová} and Stanislav {Obruča}",
title="Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium",
journal="Polymers",
year="2020",
volume="12",
number="6",
pages="1--13",
doi="10.3390/polym12061235",
url="https://www.mdpi.com/2073-4360/12/6/1235"
}
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