Evolutionary engineering approach for adaptation of PHA producing strain Halomonas halophila to levulinic and acetic acid

Evolutionary engineering approach for adaptation of PHA producing strain Halomonas halophila to levulinic and acetic acid

Abstract

Evolutionary engineering provides approaches useful for obtaining of microorganisms with desired characteristics on phenotype level (e.g. more effective growth, ability of utilization of different carbon sources, etc.). Characteristics of polyhydroxyalkanoates (PHAs), microbial biodegradable and biocompatible polymers, strongly depend on monomer composition. These materials could be advantageously used as an alternative of traditional petrochemical plastics. Evolved microbial strains obtained during evolutionary engineering experiments could be used for production of PHA with required properties for selected purposes. Promising halophilic PHA producer Halomonas halophila (CCM 3662) was exposed to microbial inhibitors commonly presented in hydrolysates of lignocellulosic biomass, namely acetic and levulinic acid. Multiple serial transfers of cell cultures in Erlenmeyer flasks after 48 hours were provided within adaptation experiments for more than 80 passages. Every passage has been basically characterized and selected preserved ones were also compared with wild-type strain considering potential of PHAs accumulation, effectivity of utilization of organic acids, testing of robustness and others features.

Evolutionary engineering provides approaches useful for obtaining of microorganisms with desired characteristics on phenotype level (e.g. more effective growth, ability of utilization of different carbon sources, etc.). Characteristics of polyhydroxyalkanoates (PHAs), microbial biodegradable and biocompatible polymers, strongly depend on monomer composition. These materials could be advantageously used as an alternative of traditional petrochemical plastics. Evolved microbial strains obtained during evolutionary engineering experiments could be used for production of PHA with required properties for selected purposes. Promising halophilic PHA producer Halomonas halophila (CCM 3662) was exposed to microbial inhibitors commonly presented in hydrolysates of lignocellulosic biomass, namely acetic and levulinic acid. Multiple serial transfers of cell cultures in Erlenmeyer flasks after 48 hours were provided within adaptation experiments for more than 80 passages. Every passage has been basically characterized and selected preserved ones were also compared with wild-type strain considering potential of PHAs accumulation, effectivity of utilization of organic acids, testing of robustness and others features.

evolutionary engineering, polyhydroxyalkanoates, Halomonas halophila, levulinic acid, acidic acid

evolutionary engineering, polyhydroxyalkanoates, Halomonas halophila, levulinic acid, acidic acid

NOVÁČKOVÁ, I.; CHATRNÁ, V.; SLANINOVÁ, E.; POŘÍZKA, J.; SEDLÁČEK, P.; OBRUČA, S.

2021

25.02.2021

1314-3530

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

35

1

United Kingdom of Great Britain and Northern Ireland

25

25

1

http://hdl.handle.net/