Publication result detail
The first insight on changes in PHA granules after extraction from extremophilic microorganisms
MRÁZOVÁ, K.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; OBRUČA, S.; KRZYŽÁNEK, V.
Original Title
The first insight on changes in PHA granules after extraction from extremophilic microorganisms
English Title
The first insight on changes in PHA granules after extraction from extremophilic microorganisms
Type
Abstract
Original Abstract
Polyhydroxyalkanoates (PHA) are microbial polyesters that are accumulated in form of intracellular granules by many prokaryotic organisms. One of the interesting properties of PHA granules is their extreme elasticity. It was found out that PHA stay elastic even at temperatures of liquid nitrogen. In the experiment, cells frozen in liquid nitrogen were freeze-fractured and imaged in a scanning electron microscope with cryostage (cryoSEM). PHA could be then observed sticking out of the fractured cells. In our work, we focused on the extraction of PHA from halophilic Halomonas halophila and thermophilic Schlegelella thermodepolymerans and subsequently on the study of changes in their morphology and elastic properties after extraction using cryoSEM.
English abstract
Polyhydroxyalkanoates (PHA) are microbial polyesters that are accumulated in form of intracellular granules by many prokaryotic organisms. One of the interesting properties of PHA granules is their extreme elasticity. It was found out that PHA stay elastic even at temperatures of liquid nitrogen. In the experiment, cells frozen in liquid nitrogen were freeze-fractured and imaged in a scanning electron microscope with cryostage (cryoSEM). PHA could be then observed sticking out of the fractured cells. In our work, we focused on the extraction of PHA from halophilic Halomonas halophila and thermophilic Schlegelella thermodepolymerans and subsequently on the study of changes in their morphology and elastic properties after extraction using cryoSEM.
Keywords
PHA, freeze-fracture, cryo SEM, Halomonas halophila, Schlegelella thermodepolymerans
Key words in English
PHA, freeze-fracture, cryo SEM, Halomonas halophila, Schlegelella thermodepolymerans
Authors
MRÁZOVÁ, K.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; OBRUČA, S.; KRZYŽÁNEK, V.
RIV year
2022
Released
13.09.2021
Book
Microscopy 2021: Conference
Edition
1
Pages from
81
Pages to
81
Pages count
1
BibTex
@misc{BUT175387,
author="Kateřina {Mrázová} and Ivana {Nováčková} and Xenie {Kouřilová} and Stanislav {Obruča} and Vladislav {Krzyžánek}",
title="The first insight on changes in PHA granules after extraction from extremophilic microorganisms",
booktitle="Microscopy 2021: Conference",
year="2021",
series="1",
edition="1",
pages="81--81",
note="Abstract"
}