Publication detail

Extremophiles – Platform strains for sustainable production of polyhydroxyalkanoates

PERNICOVÁ, I. KUČERA, D. NOVÁČKOVÁ, I. VODIČKA, J. KOVALČÍK, A. OBRUČA, S.

Original Title

Type

conference paper

Language

English

Original Abstract

Polyhydroxyalkanoates (PHA) are biodegradable polyesters, which are produced by various bacteria including numerous halophiles. Employment of halophilic strain for PHA production brings numerous benefits such as robustness of the process against contamination by ubiquitous mesophiles or possibility to isolate polymer from bacterial biomass via hypotonic lysis. In this work, we screened three moderate halophiles – Halomonas halophila, Halomonas organivorans and Halomonas salina for the presence of phaC gene encoding for PHA synthase and, subsequently, we have investigated their PHA production potential on various sugars. Among tested strains, H. organivorans demonstrated extraordinary capacity of PHA production in particular on galactose and mannose since on these saccharides PHA content in dried bacterial cells reached 83 and 90 wt. % on mannose and galactose, respectively.

Keywords

polyhydroxyalakanoates, halophiles, spent coffe grounds

Authors

PERNICOVÁ, I.; KUČERA, D.; NOVÁČKOVÁ, I.; VODIČKA, J.; KOVALČÍK, A.; OBRUČA, S.

Released

27. 5. 2019

Publisher

Trans Tech Publications Ltd

ISBN

9783035714449

Book

Materials Science Forum

Edition number

955

Pages from

Pages to

Pages count

BibTex

@inproceedings{BUT176969,
  author="Iva {Buchtíková} and Dan {Kučera} and Ivana {Nováčková} and Juraj {Vodička} and Adriána {Kovalčík} and Stanislav {Obruča}",
  title="Extremophiles – Platform strains for sustainable production of polyhydroxyalkanoates",
  booktitle="Materials Science Forum",
  year="2019",
  number="955",
  pages="74--79",
  publisher="Trans Tech Publications Ltd",
  doi="10.4028/www.scientific.net/MSF.955.74",
  isbn="9783035714449"
}