Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry

Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry

WoS Article

Interactions between hyaluronan and the antimicrobial peptide cecropin B were studied in water and PBS using high-resolution ultrasonic spectroscopy and isothermal titration calorimetry. Although each technique is fundamentally different, they both gave identical results. It was found that the molecular weight of hyaluronan plays an important role in the interactions - in particular, the transition between the rod conformation and the random coil conformation. In water, interactions were saturated in a molar charge ratio of 1.5 and not 1.0 as expected. The later saturation of the interaction probably occurred either for steric reasons or due to the interaction between functional groups in the cecropin structure, which allowed complete dissociation of the antimicrobial peptide. In PBS, in contrast to water, no interactions were observed, irrespective of the molecular weight of hyaluronan. Thus, at a sufficiently high ionic strength, the interactions were suppressed.

Interactions between hyaluronan and the antimicrobial peptide cecropin B were studied in water and PBS using high-resolution ultrasonic spectroscopy and isothermal titration calorimetry. Although each technique is fundamentally different, they both gave identical results. It was found that the molecular weight of hyaluronan plays an important role in the interactions - in particular, the transition between the rod conformation and the random coil conformation. In water, interactions were saturated in a molar charge ratio of 1.5 and not 1.0 as expected. The later saturation of the interaction probably occurred either for steric reasons or due to the interaction between functional groups in the cecropin structure, which allowed complete dissociation of the antimicrobial peptide. In PBS, in contrast to water, no interactions were observed, irrespective of the molecular weight of hyaluronan. Thus, at a sufficiently high ionic strength, the interactions were suppressed.

Hyaluronan; Cecropin B; Interaction; High-resolution ultrasonic spectroscopy; Isothermal titration calorimetry

Hyaluronan; Cecropin B; Interaction; High-resolution ultrasonic spectroscopy; Isothermal titration calorimetry

JUGL, A.; PEKAŘ, M.

2024

01.02.2023

ELSEVIER

AMSTERDAM

0141-8130

International Journal of Biological Macromolecules

227

1

Kingdom of the Netherlands

786

794

9

https://www.sciencedirect.com/science/article/pii/S014181302203046X?via%3Dihub

http://hdl.handle.net/