Laboratory Classes in Special Toxicology
FCH-MCO_SPT_PAcad. year: 2020/2021
Practical course of special toxicology to increased knowledge of special toxicology and will allow connection of theoretical and practical knowledge. Students will gain an overview of selected experimental methods used in toxicology, preparation of abiotic and biotic matrices for the determination of selected toxic compounds. Students will be introduced to a screening technique TLS (1D, 2D), methodology and evaluation of toxicity tests (test viability, cytotoxicity test). The analysis and evaluation of case studies from toxicology are included of practicum of special toxicology.
Learning outcomes of the course unit
Students will acquire new skills, knowledge and experience in these areas and increase the competence of students as follows:
Students will control the preparation of abiotic and biotic matrices for determination of selected toxic compounds.
Students will know a screening technique TLC (1D, 2D).
Students will know the methodology and principles of evaluation of toxicity tests (Test with neutral red - test viability, MTT test - cytotoxicity tests, trypan blue exclusion test - a test of viability and cytotoxicity).
Students will be able to develop their ability to analyze the acquired knowledge and to formulate objective conclusions on the basis of their assessment.
Knowledge of general toxicology, from the lectures of special toxicology of analytical chemistry and environmental chemistry,Practicum of Analytical chemistry I and II.
Recommended optional programme components
Recommended or required reading
Fenton, J.J., Toxicology, A case-Oriented Approach, CRC Press LCC, Florida, 2002
Hrdina, V. a kol., Přírodní toxiny a jedy, Galén, Praha, 2004 (CS)
Doležalová: Elearningové studijní opory, https://www.vutbr.cz/elearning/course/view.php?id=121989 (CS)
Planned learning activities and teaching methods
The course uses teaching methods in form of Laboratory exercise - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.
Assesment methods and criteria linked to learning outcomes
Evaluation includes preparation of a project and its presentation on a selected topic from the list of Case studies and all required protocols from the practical courses.
Language of instruction
1 - Introduction to practice, labor safety in laboratory of toxicology
2 - Extraction procedures for the preparation of biotic and abiotic matrices
3 - Identification of nicotine alkaloids in Nicotiana tabacum
4 - Identification of biologically active compounds (derivatives Pyranocumarin)
5 - Tropane alkaloids
6 - TLC application for PAHs separation and determination using 2D TLC
7 - Identification and quantification of toxic metals toxic metals (cadmium, arsenic) in abiotic and biotic matrices with using using polarography.
8 - Test with neutral red - test viability, MTT test - cytotoxicity tests, Test with trypane blue - test viability and cytotoxicity
9 - Application of TLC for the identification of active substances in pharmaceuticals
10 - Determination of anticoagulant rodenticide (warfarin) in traps
11 - 12 Case Study of toxicology
13 - Credit
The aim of this course is to prepare abiotic and biotic matrices for the determination of selected toxic compounds by screening techniques TLC (1D, 2D). Another objective of the course is to acquaint students with the issues of cell toxicity tests (test viability, MTT test, cytotoxicity test, cytotoxicity test). Other objectives of the subject include evaluation of case studies in the field of toxicology.
Specification of controlled education, way of implementation and compensation for absences
Practical course is supported by the eLearning courses, in which are available support materials for students, including electronic textbooks and other supplementary materials. In the second half of the semester the consultations are reserved under which it is possible to consult processing protocols. Submission of satisfactory protocols from each practical training and participation of at practices is required (minimum is 100%). Students of combined study programs will complete a practical course under the block schedule.
Classification of course in study plans
- Programme NPCP_CHTOZP Master's
branch NPCO_CHTOZP , 1. year of study, winter semester, 2 credits, compulsory
- Programme NKCP_CHTOZP Master's
branch NKCO_CHTOZP , 1. year of study, winter semester, 2 credits, compulsory
- Programme CKCP_CZV lifelong learning
branch CKCO_CZV , 1. year of study, winter semester, 2 credits, compulsory