Detail publikačního výsledku
Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food
FIALOVÁ, L.; ROMANOVSKÁ, D.; NĚMCOVÁ, A.; MÁROVÁ, I.
Original Title
Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food
English Title
Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food
Type
Abstract
Original Abstract
Certain species of red fruit, e. g. strawberries, raspberries and blueberries, are expensive components of fruit juices and purees. As a consequence, these products are subject to food fraud. The content of red fruit in them can be lower than declared, or these fruit species can be substituted for cheaper ones. This work explored and expanded a multiplex-PCR based approach to food authentication which has previously been applied to the authentication of buffalo meat products by Sakaridis et al . We successfully used triplex qPCR-HRM with amplicone detection by intercalating dye, and with primers specific for strawberry (Fragaria annanassa), red raspberry (Rubus idaeus) and berries of the Vaccinium genus to prove or disprove the presence of strawberry, red raspberry and bilberry in both model and commercial fruit purees.
English abstract
Certain species of red fruit, e. g. strawberries, raspberries and blueberries, are expensive components of fruit juices and purees. As a consequence, these products are subject to food fraud. The content of red fruit in them can be lower than declared, or these fruit species can be substituted for cheaper ones. This work explored and expanded a multiplex-PCR based approach to food authentication which has previously been applied to the authentication of buffalo meat products by Sakaridis et al . We successfully used triplex qPCR-HRM with amplicone detection by intercalating dye, and with primers specific for strawberry (Fragaria annanassa), red raspberry (Rubus idaeus) and berries of the Vaccinium genus to prove or disprove the presence of strawberry, red raspberry and bilberry in both model and commercial fruit purees.
Keywords
Multiplex PCR, qPCR-HRM, red fruit, food authentication
Key words in English
Multiplex PCR, qPCR-HRM, red fruit, food authentication
Authors
FIALOVÁ, L.; ROMANOVSKÁ, D.; NĚMCOVÁ, A.; MÁROVÁ, I.
RIV year
2021
Released
30.10.2020
ISBN
1314-3530
Periodical
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
Volume
35
Number
1
State
United Kingdom of Great Britain and Northern Ireland
Pages from
400
Pages to
400
Pages count
424
Full text in the Digital Library
BibTex
@misc{BUT167775,
author="Lenka {Fialová} and Denisa {Langová} and Andrea {Němcová} and Ivana {Márová}",
title="Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food",
year="2020",
journal="BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT",
volume="35",
number="1",
pages="400--400",
issn="1310-2818",
note="Abstract"
}