Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food

Multiplex qPCR-HRM with intercalating dye: A possible way to verify red fruit presence in food

Abstrakt

Certain species of red fruit, e. g. strawberries, raspberries and blueberries, are expensive components of fruit juices and purees. As a consequence, these products are subject to food fraud. The content of red fruit in them can be lower than declared, or these fruit species can be substituted for cheaper ones. This work explored and expanded a multiplex-PCR based approach to food authentication which has previously been applied to the authentication of buffalo meat products by Sakaridis et al . We successfully used triplex qPCR-HRM with amplicone detection by intercalating dye, and with primers specific for strawberry (Fragaria annanassa), red raspberry (Rubus idaeus) and berries of the Vaccinium genus to prove or disprove the presence of strawberry, red raspberry and bilberry in both model and commercial fruit purees.

Certain species of red fruit, e. g. strawberries, raspberries and blueberries, are expensive components of fruit juices and purees. As a consequence, these products are subject to food fraud. The content of red fruit in them can be lower than declared, or these fruit species can be substituted for cheaper ones. This work explored and expanded a multiplex-PCR based approach to food authentication which has previously been applied to the authentication of buffalo meat products by Sakaridis et al . We successfully used triplex qPCR-HRM with amplicone detection by intercalating dye, and with primers specific for strawberry (Fragaria annanassa), red raspberry (Rubus idaeus) and berries of the Vaccinium genus to prove or disprove the presence of strawberry, red raspberry and bilberry in both model and commercial fruit purees.

Multiplex PCR, qPCR-HRM, red fruit, food authentication

Multiplex PCR, qPCR-HRM, red fruit, food authentication

FIALOVÁ, L.; ROMANOVSKÁ, D.; NĚMCOVÁ, A.; MÁROVÁ, I.

2021

30.10.2020

1314-3530

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

35

1

Spojené království Velké Británie a Severního Irska

400

400

424