Course detail

Bioanalytical Methods

FCH-MC_BIMAcad. year: 2015/2016

Lectures are focused on analysis of nucleic acids (mainly DNA). The lectures are focused on the explanation of methods which are used for DNA isolation, evaluation the quality of extracted DNA, DNA amplification (PCR) and results evaluation. The different molecular biological techniques (restriction analysis, DNA/DNA hybridisation, sequencing) and DNA sequence analysis are discussed.

Language of instruction

Czech

Number of ECTS credits

4

Mode of study

Not applicable.

Guarantor

doc. Ing. Bohuslav Rittich, CSc.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Learning outcomes of the course unit

Theoretical aspects of various experimental techniques of DNA analysis

Prerequisites

The lectures are followed by Laboratory course of molecular biotechnology

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Examination is oral

Course curriculum

1. Methods of cell analysis. Application of chromatographic methods in microorganisms identification.
2. Isolation of nucleic acids. Spectrophotometric evaluation of DNA quality.
3. Separation of nucleic acids by electromigration and chromatographic methods.
4. DNA fragmentation by restriction enzymes. Artificial restriction systems.
5. Polymerase Chain Reaction (PCR).
6. Chemical synthesis of DNA.
7. Other amplification methods.
8. Distinguishing of live and dead cells.
9. Carriers with immobilized ligands in molecular diagnostics.
10. DNA/DNA hybridization and DNA probes.
11. Fluorescence in situ hybridization - FISH. Chips
12.Sequencing of nucleic acids.
13. Bioinformatics (sequences analysis).

Work placements

Not applicable.

Aims

Lectures cover methodologies commonly used in DNA research

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is not obligatory but it is recommended

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Not applicable.

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme NPCP_CHTP Master's

    branch NPCO_CHTP , 2 year of study, winter semester, compulsory-optional

  • Programme NKCP_CHTP Master's

    branch NKCO_CHTP , 2 year of study, winter semester, compulsory-optional

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1 year of study, winter semester, compulsory-optional

Type of course unit

 

Lecture

26 hod., optionally

Teacher / Lecturer

doc. Ing. Bohuslav Rittich, CSc.
doc. RNDr. Alena Španová, CSc.
Ing. Štěpánka Trachtová, Ph.D.