Course detail

Instrumental and structure analysis

FCH-MC_ISAAcad. year: 2015/2016

Fundamentals, instrumentation and usage of methods of chemical, physical and structure analysis. Separation methods: chromatography methods-gas chromatography, liquid chromatography, supercritical fluid chromatography; electrophoresis, isotachophoresis. Electroanalysis: special voltammetry.Thermal analysis: thermogravimetry, differential thermal analysis, differential scanning calorimetry. Atomic spectrometry: Atomic emission spectrometry, atomic absorption spectrometry, atomic fluorescence spectrometry. X-ray fluorescence spectrometry. Mass spectrometry organic and inorganic. Infrared and Raman spectroscopy. Nuclear magnetic resonance spectroscopy. Electron spin resonance spectroscopy. Moessbauer spectrometry. Photoelectron spectroscopy (ESCA, Auger spectroscopy). Microbeam and surface analysis: Photon probe techniques, electron probe techniques, ion probe techniques(SEM, TEM, EPXMA,SIMS,FIM,STM, AFM). X-ray diffraction. Laser in analytical chemistry. Hyphenated techniques.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

prof. Ing. Josef Čáslavský, CSc.

Department

Institute of Chemistry and Technology of Environmental Protection (ÚCHTOŽP)

Learning outcomes of the course unit

Completion of this course will increase students' competence in the following way:
a) Students will know the principles of instrumental analytical methods used in routine analytical practice
b) Students will master the methods of interpretation of the obtained measurement results both in qualitative and in quantitative terms
c) Students will be able to creatively optimize the selection of analytical techniques in solving specific analytical problem

Prerequisites

Basic knowladge of physics, general chemistry, physical chemistry, inorganic, organic, analytical chemistry and biochemistry.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Oral examination within the range of discussed topics. Assessed is the knowledge of the principles of analytical methods including their specifics and creative ability of solution of given analytical problem.

Course curriculum

1st-3rd week: Separation methods
Chromatography - gas chromatography, liquid chromatography, supercritical fluid chromatography, ion chromatography;
Electromigration techniques: electrophoresis, isotachophoresis, isoelectric focusation, micellar electrokinetic capillary chromatography, gel electrophoresis, capillary electrochromatography.

4th-6th week: Molecular spectrometry
Infrared spectrometry, Raman spectrometry, UV-VIS spectrophotometry, luminescence.

7th-8th week: Atomic spectrometry - Atomic emission spectrometry, atomic absorption spectrometry, atomic fluorescence spectrometry.

9th-11th week: Mass spectrometry (principles, instrumentation, introduction to MS spectra interpretation). Hyphenated techniques.

12th-13th week: Nuclear magnetic resonance, electron spin resonance.

Work placements

Not applicable.

Aims

The aim of this course is:
- to familiarize students with selected instrumental analytical methods used in the analytical practice
- to give them a thorough overview of the basic characteristics of these methods
- to teach students how to choose the optimal instrumental method for the concrete analytical problem

Specification of controlled education, way of implementation and compensation for absences

none

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Kellner R., Mermet J. M., Otto M., Widmer H. M.: Analytical Chemistry. Willey-VCH, Weinheim 1998. (EN)
Sommer L.: Teoretické základy analytické chemie I., II., III. VUT Brno, Brno 1995. (CS)

Type of course unit

Lecture

26 hod., optionally

Teacher / Lecturer

prof. Ing. Josef Čáslavský, CSc.