Czech

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Theoretical aspects of various experimental techniques used in molecular biotechnology research

Pass out the lectures of Molecular genetics I and II is recomended

Not applicable.

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Examination is oral

1. Relationship between chemical production and biotechnologies.
2. Transfer of genes using recombinant DNA technology.
3. Cloning vectors and their use.
4. Transformation of cells by foreign DNA. Selection of cells with cloned gene.
5. Gene libraries. Cloning of sequences coding eucaryotic proteins.
6. Manipulation of gene expression in prokaryotes.
7. Gene expression from strong and inducible promotors.
8. Fusion proteins. Translation expression vectors. Increasing of protein stability. Integration and shuttle vectors.
9. Production of heterologous proteins in eucaryotic cells.
10.Yeast expression systems.
11. Insect and mammal expression systems.
12. Transgenic plants, their construction and use.
13. Transgenic animals, thein construction and use.

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Lectures cover methodologies commonly used in molecular biotechnology in construction of transgenic organims.

Attendance at lectures is not obligatory but it is recommended

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