Course detail
Special molecular biotechnology
FCH-DC_SMBAcad. year: 2021/2022
Not applicable.
Language of instruction
Czech
Number of ECTS credits
0
Mode of study
Not applicable.
Guarantor
Learning outcomes of the course unit
Not applicable.
Prerequisites
Not applicable.
Co-requisites
Not applicable.
Planned learning activities and teaching methods
Not applicable.
Assesment methods and criteria linked to learning outcomes
Not applicable.
Course curriculum
Basic methods of recombinant DNA technology: isolation and characterization of DNA and RNA, restriction endonucleases and other enzymes, cloning, polymerase chain reaction, gene mapping, methods of transformation, transformation vectors - plasmids, phages, cosmids, YAC. Identification of recombinant DNA. Gene expression in microbial (bacteria, yeast) and animal expression systems, markers. Identification and purification of recombinant proteins (PAGE-SDS, Western blot, immunodetection).
Work placements
Not applicable.
Aims
Not applicable.
Specification of controlled education, way of implementation and compensation for absences
Not applicable.
Recommended optional programme components
Not applicable.
Prerequisites and corequisites
Not applicable.
Basic literature
Glick B. R., Pasternak J. J.: Molecular Biotechnology. ASM Press, Washington 2003. (EN)
J. Čechová, J. Coufal, E.B. Jagelská, M. Fojta, V. Brázda, P73, Like Its P53 Homolog, Shows Preference for Inverted Repeats Forming Cruciforms, PLoS One. 13 (2018). doi:10.1371/journal.pone.0195835. (EN)
https://www.thermofisher.com/cz/en/home/life-science/cloning/gateway-cloning.html (EN)
J. Čechová, J. Coufal, E.B. Jagelská, M. Fojta, V. Brázda, P73, Like Its P53 Homolog, Shows Preference for Inverted Repeats Forming Cruciforms, PLoS One. 13 (2018). doi:10.1371/journal.pone.0195835. (EN)
https://www.thermofisher.com/cz/en/home/life-science/cloning/gateway-cloning.html (EN)
Recommended reading
Not applicable.