Course detail
Bioengineering II
FCH-MC_BIO2Acad. year: 2015/2016
Lectures are focused on explanation of basic operations (cell disruption, protein precipitation, exctraction, large-scale chromatography) used in isolation and purification of biologically active compounds (proteins, nucleic acids) from microorganisms.
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2. Systems with immobilized cells and enzymes.
3. Enzymatic catalysis in anhydrous and quasi anhydrous solvents.
4. Properties of fluids.
5. Mechanical, chemical and enzymatic cell disruption.
6. Precipitation and salting-out odf proteins.
7. Filtration. Centrifugation.
8. Extraction.
9. Extraction using aqueous two-phase systems.
10. Types of extractors and their design.
11. Large scale chromatographic techniques.
12. Drying. Freeze-drying. Protein stability.
13. Validation of production quality.
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