Course detail

Biochemistry II

FCH-MA_BCH2Acad. year: 2021/2022

Metabolic pathways. Bioenergetics and oxidative metabolism. Short-term and long-term control mechanisms - regulation of enzyme activity, gene expression regulation; cell signaling. Carbohydrate metabolism - major metabolic pathways and their control, special pathways. Tricarboxylic acid cycle. Electron transport chain. Oxidative phosphorylation. Lipid metabolism - utilization and storage of energy. Metabolismus of nitrogen compounds I - Amino acid metabolism. Metabolsim of nitrogen compounds II - Porfyrin and heme metabolism, Nucelotide metabolism. Metabolic interrelationships and control. Compartmentation. Membrane transport. Biochemistry of hormones and the hormonal cascade system.

Language of instruction

English

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

prof. RNDr. Ivana Márová, CSc.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Offered to foreign students

Of all faculties

Learning outcomes of the course unit

The course Biochemistry II is focused on detailed study of metabolic pathways and their regulation on molecular as well as cellular level. The knowlege obtained after passing the course can serve as basis for further studies, especially for biotechnology, molecular biology and bioengineering.

Prerequisites

Examination - Biochemistry I.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Oral examination, combination of 3 questions, evaluation according to ECTS standards.

Exercises: active participation in round discussions, individual presentation, 4 tests /10 questions, minimum points - 70 %.

Course curriculum

The aim of the course is to acquaint students with the basics of cellular metabolism and its regulation. Based on the general principles of biochemical thermodynamics, the basic pathways of metabolism of carbohydrates, fatty acids and amino acids will be discussed. The course also includes biological oxidation, cellular energy production and photosynthesis. Within the metabolism of nucleic acids and proteins, the course of gene expression and its regulation will be explained. The course also includes mutual relations and regulation within the intermediate metabolism of nutrients.
During exercises will be discussed individual topics presented in lectures. Round discussions will be realized and completed by indiviual presentations of students.

1. Basic principles of metabolism - overview.
2. Metabolic control mechanisms I - regulation of enzyme activity, cell signaling.
3. Metabolic control mechanisms I I - regulation of gene expression, protein turnover.
4. Carbohydrate metabolism - glycolysis, fermentation.
5. Metabolsim of fructose and galactose. Glycogene metabolism and its control. Hormones.
6. Tricarboxylic ycid cycle. Glyoxalate cycle. Glucoenogenesis.
7. Metabolism of lipids and fatty acids. The ketone bodies.
8. Biological oxidations. Electron transport chain and oxidative phosphorylation. Photosynthesis.
9. Isoprenoid pathway. Cholesterol metabolis.
10. Nitrogen metabolism I - fixation, metabolism of amino acids, the urea cycle.
11. Nitrogen metabolism II - porphyrins, heme metabolism. Nucleotides metabolism.
12. Secondary metabolism - overview.

Work placements

Not applicable.

Aims

The aim of the course Biochemistry II is to introduce basic principles of cell metabolism and its regulation. Based on general thermodynamic principles the course of central biochemical pathways including metabolism of saccharides, fatty acids and amino acids is elucidated. Further, basic principles of biological oxidations, cell energy production and photosynthesis are discussed. Next, metabolism of nucleic acids and proteins and processes involved in gene expression are introduced. As a part of course intermediate metabolism and complex regulation of metabolic pathways are presented.

Specification of controlled education, way of implementation and compensation for absences

Recommended.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Stryer L.: Biochemistry. 9th Edition, Palgrave, 2019. (EN)
Voet D. (2011): Biochemistry, John Wiley And Sons Ltd, ISBN-13: 978-0470570951 (EN)