Course detail

Bioanalytical Methods

FCH-MC_BIMAcad. year: 2023/2024

Not applicable.

Language of instruction


Number of ECTS credits


Mode of study

Not applicable.

Entry knowledge

Not applicable.

Rules for evaluation and completion of the course

Examination is oral
Attendance at lectures is not obligatory but it is recommended


The aim is to acquire knowledge about the methods that are used for analysis of biological materials.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Záruba, Kamil. Analytická chemie. Praha: VSCHT v Praze, 2016. (CS)
Manz, A. Bioanalytical chemistry. London: Imperial College Press, 2015. (EN)

Recommended reading

UGO, Paolo and Coll. Bioanalytical Chemistry. De Gruyter, 2021. (EN)
Ramesh, Vasudevan. Biomolecular and Bioanalytical Techniques. Wiley, 2019. (EN)


Classification of course in study plans

  • Programme NPCP_PCHBT Master's, 2. year of study, winter semester, compulsory-optional

  • Programme NPCP_CHMA Master's

    specialization CHBL , 2. year of study, winter semester, compulsory-optional
    specialization BF , 2. year of study, winter semester, compulsory-optional

  • Programme NKCP_CHPL Master's, 2. year of study, winter semester, compulsory-optional
  • Programme NPCP_CHPL Master's, 2. year of study, winter semester, compulsory-optional

Type of course unit



26 hours, optionally

Teacher / Lecturer


1) Introduction to bioanalytical methods and their application
2) Overview of bioanalytical techniques, basic principles of biopolymer analysis
3) Enzymology - Enzyme distribution, enzyme kinetics, enzymes as analytical reagents
4) Enzymology - immobilization of enzymes, use in practice
5) Immunochemical Methods - Principles and Overview of Antibody Techniques
6) Immunochemical methods - fluorescence, bioluminescence, radioactive and enzyme immunoassay
7) Nanotechnology, biosensors, construction and applications
8) Chromatographic techniques - gel permeation, affinity and ion exchange chromatography, gas (GC) and liquid chromatography (HPLC, UHPLC), supercritical fluid chromatography (SFC) and their use in biomolecular analysis
9) Spectrophotometric and microscopic methods - optical, fluorescence, infrared, raman and high-frequency spectroscopy, nuclear magnetic resonance, atomic absorption and emission spectrometry
10) Electrophoretic methods - electrophoresis, isoelectric focusing, isotachophoresis, affinity, two-dimensional, zonal and capillary electrophoresis.
11) Molecular techniques, polymerase chain reaction (PCR), RT-PCR detection of specific product sequences in situ, DNA and RNA chips, examples of practical use
12) Biological and microbiological analytical methods, flow cytometry, fluorescence techniques, electron microscopy
13) Modern bioanalytical techniques

Guided consultation in combined form of studies

26 hours, optionally

Teacher / Lecturer