Course detail
Laboratory Classes in Instrumental and Structure Analysis - FSBT
FCH-MC_ISA_PAcad. year: 2023/2024
Practical class to the lecture Instrumental and Structure Analysis.
Students are familiarized with instrumental analytical techniques used by control and analysis of natural substances during this class. The course practically develops knowledge from the subjects Analytical Chemistry II and Instrumental and Structural Analysis. Individual tasks and laboratory equipment were chosen so that students come into direct contact with modern infrastructure in solving specific problems of analysis of samples of natural substances. Practical exercises are focused on separation techniques (chromatography and electrophoresis), elemental analysis, spectral and structural analysis.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Entry knowledge
Rules for evaluation and completion of the course
The full attendance is required. In the case of absence, the particular seminar can be compensated by attending with another group or in the last week of semester. An unexcused absence is a reason for the loss of the course-unit credit.
The examination of basic knowledge relating to the particular project is done at the beginning of each exercise. Each student will write out laboratory diary. Results from the practise will be summarized as hand-writen laboratory protocol, which will be part of laboratory diary.
Laboratory reports are required till 7 days after the last exercise. Classification of the student will be based on evaluation of laboratory diary, protocols and activity in laboratory.
Aims
Graduates of this practical subject will acquire basic knowledge about selected instrumental analytical methods which they can may encounter in food chemistry practice. Student will learn basics of operation, maintanance and fuction principles of specific instruments. Furthermore acquire the skills of data evalutaion.
Study aids
Prerequisites and corequisites
Basic literature
Nováková L., Douša M. a kol.: Moderní HPLC separace v teorii a praxi I, Europrint, Praha, Česká republika, 2013. (CS)
Nováková L., Douša M. a kol.: Moderní HPLC separace v teorii a praxi II, Europrint, Praha, Česká republika, 2013. (CS)
Mikuš P., Piešťanský J., Dokupilová S.: Kvapalinová chromatografia, hmotnosná spektrometria, Veda, Bratislava, Slovensko, 2018. (SK)
Milata V.: Vybrané metódy molekulovej spektroskopie, STU, Bratislava, Slovensko, 2007. (SK)
Kanický V., Kučera J., Tomášek V.: Analýza anorganických látek, 2THETA, Český Těšín, 2021. (CS)
Recommended reading
Robinson J.W. a kol.:Instrumental Analytical Chemistry: An Introduction, Taylor & Francis Ltd, London, UK, 2017. (EN)
Pool C.: Capillary elecromigraion separation methods, Elsevier, Amsterdam, Holandsko, 2018. (EN)
Elearning
Classification of course in study plans
- Programme NKCP_CHPL Master's 1 year of study, winter semester, compulsory
- Programme NPCP_CHPL Master's 1 year of study, winter semester, compulsory
- Programme NPCP_PCHBT Master's 1 year of study, winter semester, compulsory
Type of course unit
Guided consultation in combined form of studies
Teacher / Lecturer
Laboratory exercise
Teacher / Lecturer
Syllabus
1. Optical emmision spectrometry with inductively coupled plasma - ICP-OES
2. Mass spectrometry with inductively coupled plasma - ICP-MS
3. Capillary zone electrophoresis - CZE
4. Chip gel electrophoresis - CGE
5. Liquid chromatography - HPLC
6. Liquid chromatography with mass detection - LC-MS
7. Gas chromatography - GC
8. Gas chromatography with mass detection - GC-MS
9. UV-VIS Spectrophotometry - UV-VIS
10. Infrared spectroscopy – IR
Elearning