Course detail
Laboratory Classes in Instrumental Analysis - S2
FCH-MC_IA2_PAcad. year: 2023/2024
The subject aims at practical demostration of analytical techniques which are used in the analysis of materials, biological systems, transport systems for biologically active substances and in technical practice.
Language of instruction
Czech
Number of ECTS credits
3
Mode of study
Not applicable.
Guarantor
Entry knowledge
Knowledge of general, inorganic, organic, physical, analytical chemistry and biochemistry.
Rules for evaluation and completion of the course
The resulting evaluation is the average of the evaluation of individual protocols.
Mandatory participation in all blaboratory classes.
Mandatory participation in all blaboratory classes.
Aims
The aim of the subject is Improvement of knowledge gained during lectures and familiarize students with methods of instrumental and structural analysis used in the field of chemistry of biologically active substances.
Gaining practical knowledge in the field of analysis of biological samples, selection of the apropriate method, evaluation and interpretation of the results.
Gaining practical knowledge in the field of analysis of biological samples, selection of the apropriate method, evaluation and interpretation of the results.
Study aids
Not applicable.
Prerequisites and corequisites
Not applicable.
Basic literature
Klouda P.: Moderní analytické metody. Nakladatelství Pavel Klouda, Ostrava 2003. (CS)
Študlík a kolektiv: Analytické separační metody. Nakladatelství Karolinum, Praha 2004 (CS) (CS)
Králová a kolektiv: Bioanalytické metody. Vysoká škola chemicko-technologická v Praze (CS) (CS)
Študlík a kolektiv: Analytické separační metody. Nakladatelství Karolinum, Praha 2004 (CS) (CS)
Králová a kolektiv: Bioanalytické metody. Vysoká škola chemicko-technologická v Praze (CS) (CS)
Recommended reading
Not applicable.
eLearning
eLearning: currently opened course
Type of course unit
Guided consultation in combined form of studies
26 hours, obligation not entered
Teacher / Lecturer
Laboratory exercise
39 hours, compulsory
Teacher / Lecturer
Ing. Miloš Dvořák, Ph.D.
Ing. Martin Gajdušek
Ing. Martina Havlíková
Ing. Zuzana Juglová
Ing. Jitka Krouská, Ph.D.
doc. RNDr. Renata Mikulíková, Ph.D.
doc. Ing. Filip Mravec, Ph.D.
Ing. Jakub Nábělek
Ing. Jan Obračaj
Ing. Jaromír Pořízka, Ph.D.
doc. Ing. Petr Sedláček, Ph.D.
Mgr. Zuzana Šedrlová
Ing. Štěpánka Trachtová, Ph.D.
Ing. Martin Gajdušek
Ing. Martina Havlíková
Ing. Zuzana Juglová
Ing. Jitka Krouská, Ph.D.
doc. RNDr. Renata Mikulíková, Ph.D.
doc. Ing. Filip Mravec, Ph.D.
Ing. Jakub Nábělek
Ing. Jan Obračaj
Ing. Jaromír Pořízka, Ph.D.
doc. Ing. Petr Sedláček, Ph.D.
Mgr. Zuzana Šedrlová
Ing. Štěpánka Trachtová, Ph.D.
Syllabus
1. Introduction
2. Liquid chromatography for analysis of medical drugs
3. LC-MS analysis of biological samples
4. Analysis of volatile compounds by gas chromatography
5. UV-VIS spectroscopy as a tool for analysis of enzymes
6. FTIR spectroscopy for analysis of biopolymers
7. Fluorescence spectroscopy as method for analysis of transport of biologically active samples
8. Fluoresecence microscopy analysis at single cell level
9. Gel electrophoresis
10. Determination of fermentation products by CZE
11. Analysis of heavy meatl contamination by ICP-MS
12. Flow cytometry to analysis of cell populations
13. ELISA instrumentace and assays
eLearning
eLearning: currently opened course