Course detail
Laboratorry Classes in Microbiology
FCH-BC_MIB_PAcad. year: 2024/2025
The basic rules of safety work in microbiological laboratory. Preparation of culture media, various sterilization methods. Inoculation, incubation, isolation of microorganisms. Preparation of smears for staining, microscopic observation. The Gram´s staining method, several other special staining techniques. Quantification techniques - direct and indirect method. Yeasts. Fungi. The microbiological analysis of water and soil. The microbiological control of foods.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Entry knowledge
Rules for evaluation and completion of the course
Attendance is required and checked by the teacher.
Aims
Practical knowledge and skills of basic microbiological laboratory methods: preparation of culture medium, inoculation, incubation and isolation of microorganisms, preparation of smears for staining, various staining methods, microscopic observation of microorganisms.
The ability to apply these skills for the microbiological examination of foods, water and other materials.
Study aids
Prerequisites and corequisites
- compulsory prerequisite
Laboratory Classes in Inorganic Chemistry I
Basic literature
Němec, M., Horáková, D.: Základy mikrobiologie. Vydavatelství MU, Brno 1999. (CS)
Recommended reading
Horáková, K., Baráthová, H., Vollek, V.: Mikrobiológia - návody na cvičenie. Slovenská vysoká škola technická v Bratislave, Bratislava 1986. (CS)
Elearning
Classification of course in study plans
- Programme BPCP_CHTPO Bachelor's
specialization CHPL , 3 year of study, winter semester, compulsory
specialization PCH , 3 year of study, winter semester, compulsory
specialization BT , 3 year of study, winter semester, compulsory - Programme BKCP_CHTPO Bachelor's
specialization PCH , 3 year of study, winter semester, compulsory
specialization BT , 3 year of study, winter semester, compulsory
specialization CHPL , 3 year of study, winter semester, compulsory - Programme BKCP_AAEFCH Bachelor's 2 year of study, winter semester, compulsory-optional
- Programme BPCP_AAEFCH Bachelor's 2 year of study, winter semester, compulsory-optional
- Programme BPCP_CHMA Bachelor's 3 year of study, winter semester, compulsory-optional
- Programme BPCP_CHTP Bachelor's
specialization CHPL , 3 year of study, winter semester, compulsory
specialization PCH , 3 year of study, winter semester, compulsory
specialization BT , 3 year of study, winter semester, compulsory - Programme BKCP_CHTP Bachelor's
specialization CHPL , 3 year of study, winter semester, compulsory
specialization PCH , 3 year of study, winter semester, compulsory
specialization BT , 3 year of study, winter semester, compulsory
Type of course unit
Laboratory exercise
Teacher / Lecturer
Syllabus
1. Preparation of sterile glassware and other tools
2. Preparation of culture media and its sterilization
3. Isolation, inoculation and storage of microorganisms. Cultivation conditions
4. Microscopic observation of microorganisms.
5. The negative staining, staining of bacterial capsules and spores
6. Quantification of microorganisms by direct microscopic counts. Vital staining.
7. Quantification of microorganisms by colony count method.
8. Morphology of yeasts
9. The microbiological analysis of water
10. Morphology of fungi
11. The microbiological analysis of soil
12. The microbiological control of foods
Elearning