Course detail

Laboratorry Classes in Microbiology

FCH-BC_MIB_PAcad. year: 2023/2024

The basic rules of safety work in microbiological laboratory. Preparation of culture media, various sterilization methods. Inoculation, incubation, isolation of microorganisms. Preparation of smears for staining, microscopic observation. The Gram´s staining method, several other special staining techniques. Quantification techniques - direct and indirect method. Yeasts. Fungi. The microbiological analysis of water and soil. The microbiological control of foods.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

Ing. Štěpánka Trachtová, Ph.D.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Entry knowledge

Successful completion of practical classes is conditional on the basic theoretic knowledge of general microbiology.

Rules for evaluation and completion of the course

Conditions for awarding the course unit credit: 100 % participation in laboratory classes, elaboration and submitting of reports in written form and credit test (at minimum 70 %).
Attendance is required and checked by the teacher.

Aims

The aim of these practical classes is to make students familiar with basic procedures, methods and techniques used in microbiological laboratory.
Practical knowledge and skills of basic microbiological laboratory methods: preparation of culture medium, inoculation, incubation and isolation of microorganisms, preparation of smears for staining, various staining methods, microscopic observation of microorganisms.
The ability to apply these skills for the microbiological examination of foods, water and other materials.

Study aids

Not applicable.

Prerequisites and corequisites

compulsory prerequisite
- Laboratory Classes in Inorganic Chemistry I

Basic literature

Drdák M., Veselá M.: Praktikum z obecné mikrobiologie. FCH VUT v Brně, Brno 1998. (CS)
Němec, M., Horáková, D.: Základy mikrobiologie. Vydavatelství MU, Brno 1999. (CS)

Type of course unit

Laboratory exercise

39 hours, compulsory

Teacher / Lecturer

Ing. Iva Buchtíková, Ph.D.
Ing. Lenka Fialová, Ph.D.
Radka Nováková
Ing. Štěpánka Trachtová, Ph.D.
doc. Ing. Eva Vítová, Ph.D.
Ing. Štěpánka Trachtová, Ph.D.

Syllabus

The basic rules of safety work in microbiological laboratory.
1. Preparation of sterile glassware and other tools
2. Preparation of culture media and its sterilization
3. Isolation, inoculation and storage of microorganisms. Cultivation conditions
4. Microscopic observation of microorganisms.
5. The negative staining, staining of bacterial capsules and spores
6. Quantification of microorganisms by direct microscopic counts. Vital staining.
7. Quantification of microorganisms by colony count method.
8. Morphology of yeasts
9. The microbiological analysis of water
10. Morphology of fungi
11. The microbiological analysis of soil
12. The microbiological control of foods

eLearning

eLearning: currently opened course