Latest improvements in development of simple splitless gradient liquid chromatographic system for micro and nano columns

Latest improvements in development of simple splitless gradient liquid chromatographic system for micro and nano columns

Abstrakt

Our simple liquid chromatographic system for splitless gradient nanocolumn separations at backpressure up to 50 bars was modified to run analysis under the pressure up to 300 bars. Programmable syringe pump was equipped with a 250-ul high-pressure syringe and mobile phase gradients were created and stored in relatively long capillary. Available linear gradients of volumes from 5 to 50 ul traced by uracil are presented. Sample introduction was performed as separate step and real large volume of sample (tens of microliters) could be injected and pre-concentrated on-column. Relative standard deviation of retention times and peak areas were received (RSD < 0.3 % and RSD < 5 % respectively).

Our simple liquid chromatographic system for splitless gradient nanocolumn separations at backpressure up to 50 bars was modified to run analysis under the pressure up to 300 bars. Programmable syringe pump was equipped with a 250-ul high-pressure syringe and mobile phase gradients were created and stored in relatively long capillary. Available linear gradients of volumes from 5 to 50 ul traced by uracil are presented. Sample introduction was performed as separate step and real large volume of sample (tens of microliters) could be injected and pre-concentrated on-column. Relative standard deviation of retention times and peak areas were received (RSD < 0.3 % and RSD < 5 % respectively).

liquid chromatography, splitless gradient

liquid chromatography, splitless gradient

ŠESTÁK, J.; KAHLE, V.; DUŠA, F.

2014

15.06.2013

Amsterdam

HPLC 2013 Amsterdam, 39th International Symposium on High-Performance-Liquid-Phase Separations and Related Techniques 16-20 June 2013, Book of Abstracts

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