Detail publikačního výsledku

Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns

ŠESTÁK, J.; KAHLE, V.

Originální název

Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns

Anglický název

Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns

Druh

Článek WoS

Originální abstrakt

In this short communication, a low cost and easily feasible pressure-controlled extension of the previously described simple gradient liquid chromatography platform is proposed. A test mixture of four nitro esters was separated by 10-60%v/v acetone/water gradient and a high repeatability of retention volumes at 20 MPa (RSD less than 0.45%) was realized. Separations were also performed at different values of pressure (20, 25, and 31 MPa), and only small variations of the retention volumes (up to 0.8%) were observed. In this particular case, the gain in the analysis speed of 7% compared to the constant flow mode was realized at a constant pressure.

Anglický abstrakt

In this short communication, a low cost and easily feasible pressure-controlled extension of the previously described simple gradient liquid chromatography platform is proposed. A test mixture of four nitro esters was separated by 10-60%v/v acetone/water gradient and a high repeatability of retention volumes at 20 MPa (RSD less than 0.45%) was realized. Separations were also performed at different values of pressure (20, 25, and 31 MPa), and only small variations of the retention volumes (up to 0.8%) were observed. In this particular case, the gain in the analysis speed of 7% compared to the constant flow mode was realized at a constant pressure.

Klíčová slova

Constant pressure HPLC; Gradient elution; Simple liquid chromatograph; Nano columns; Nitro esters

Klíčová slova v angličtině

Constant pressure HPLC; Gradient elution; Simple liquid chromatograph; Nano columns; Nitro esters

Autoři

ŠESTÁK, J.; KAHLE, V.

Rok RIV

2015

Vydáno

11.06.2014

ISSN

0021-9673

Periodikum

JOURNAL OF CHROMATOGRAPHY A

Svazek

1350

Číslo

6

Stát

Nizozemsko

Strany od

68

Strany do

71

Strany počet

4

BibTex

@article{BUT107541,
  author="Jozef {Šesták} and Vladislav {Kahle}",
  title="Constant pressure mode extended simple gradient liquid chromatography system for micro and nano columns",
  journal="JOURNAL OF CHROMATOGRAPHY A",
  year="2014",
  volume="1350",
  number="6",
  pages="68--71",
  issn="0021-9673"
}