DLS Microrheology of biopolymer solution, Probe size effect

DLS Microrheology of biopolymer solution, Probe size effect

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Structure of biopolymers plays important role for its industrial applications and affect their potential usability. The more information about the micro and macrostructure of the sample the better is evaluation of potential behavior of the sample and suitability for use. For microstructure determination we used passive microrheology method by utilizing diffusing light spectroscopy device and microrheology probes to characterize properties of the sample. Three different probes with unlike particle surfaces were chosen to determine the most appropriate one for biopolymer solutions measurements. To estimate possible probe – biopolymer interactions, which are undesirable for microrheological measurements, zeta potential of particles in the solutions was studied. Results from DLS microrheology were compared with FCS nenorheology measurements. Our data show importance of appropriate particle choice, with an emphasis on the particle surface chemistry. The most suitable particles for biopolymer solutions are polystyrene particles, or PS-COOH particles that avoid particle – biopolymer interaction. DLS and FCS microrheology data exhibit good agreement in wide time range.

Structure of biopolymers plays important role for its industrial applications and affect their potential usability. The more information about the micro and macrostructure of the sample the better is evaluation of potential behavior of the sample and suitability for use. For microstructure determination we used passive microrheology method by utilizing diffusing light spectroscopy device and microrheology probes to characterize properties of the sample. Three different probes with unlike particle surfaces were chosen to determine the most appropriate one for biopolymer solutions measurements. To estimate possible probe – biopolymer interactions, which are undesirable for microrheological measurements, zeta potential of particles in the solutions was studied. Results from DLS microrheology were compared with FCS nenorheology measurements. Our data show importance of appropriate particle choice, with an emphasis on the particle surface chemistry. The most suitable particles for biopolymer solutions are polystyrene particles, or PS-COOH particles that avoid particle – biopolymer interaction. DLS and FCS microrheology data exhibit good agreement in wide time range.

Diffusing light spectroscopy, microrheology, microstructure, zeta potential, fluorescence correlation spectroscopy

Diffusing light spectroscopy, microrheology, microstructure, zeta potential, fluorescence correlation spectroscopy

HNYLUCHOVÁ, Z.; MONDEK, J.; PEKAŘ, M.

05.11.2014

978-80-87294-55-0

Nanocon 2014 Conference Proceedings

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http://hdl.handle.net/