Detail publikačního výsledku

TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA

Čuta R., Fričová M., Rittich B., Španová A.

Originální název

TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA

Anglický název

TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA

Druh

Konferenční sborník (ne stať)

Originální abstrakt

The aim of this work was the optimization of procedure the crude cell lysates preparation of Lactobacillus genus. In this experiment different lysis methods were tested: buffer B, 4% solution of washing powder Amway and using of immobilized lysozyme. At the same time a lysing reagent was tested. As a control sample was used a buffer A which contained no lysozyme. Crude lysates were used for a DNA isolation using magnetic microspheres. The DNA was examined by the PCR method.

Anglický abstrakt

The aim of this work was the optimization of procedure the crude cell lysates preparation of Lactobacillus genus. In this experiment different lysis methods were tested: buffer B, 4% solution of washing powder Amway and using of immobilized lysozyme. At the same time a lysing reagent was tested. As a control sample was used a buffer A which contained no lysozyme. Crude lysates were used for a DNA isolation using magnetic microspheres. The DNA was examined by the PCR method.

Klíčová slova

DNA, lysis, PCR

Klíčová slova v angličtině

DNA, lysis, PCR

Autoři

Čuta R., Fričová M., Rittich B., Španová A.

Vydáno

01.10.2014

ISBN

978-80-904959-2-0

Strany od

170

Strany do

171

Strany počet

2

Plný text v Digitální knihovně

http://hdl.handle.net/

BibTex

@proceedings{BUT111852,
  editor="Robert {Čuta} and Michaela {Čutová} and Bohuslav {Rittich} and Alena {Španová}",
  title="TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA",
  year="2014",
  pages="170--171",
  isbn="978-80-904959-2-0"
}