Detail publikačního výsledku

Immunomagnetic capture and preconcentration of Lactobacillus cells

RITTICH, B.; TURKOVÁ, K.; TRACHTOVÁ, Š.; VAŇÁSEK, J.; HORÁK, D.; KOZÁKOVÁ H.; ŠPANOVÁ A.

Originální název

Immunomagnetic capture and preconcentration of Lactobacillus cells

Anglický název

Immunomagnetic capture and preconcentration of Lactobacillus cells

Druh

Abstrakt

Originální abstrakt

The aim of this work was to prepare and evaluate imunomagnetic microspheres for the isolation of Lactobacillus cells from food matrices. Two types of magnetic particles coated by streptavidine were used: magnetic porous glass (MPG) (diameter 5 µm) (10 mg/mL, 4.6 x 107 particles/mL) (PureBiotech, USA) and 2.9 µm magnetic poly(glycidyl methacrylate) microspheres containing NH2 groups [P(GMA)-NH2]. They were functionalised with biotynilated anti-Lactobacillus antibody and used for separation of Lactobacillus cells. Preconcentration of cells in a milk product was also tested.

Anglický abstrakt

The aim of this work was to prepare and evaluate imunomagnetic microspheres for the isolation of Lactobacillus cells from food matrices. Two types of magnetic particles coated by streptavidine were used: magnetic porous glass (MPG) (diameter 5 µm) (10 mg/mL, 4.6 x 107 particles/mL) (PureBiotech, USA) and 2.9 µm magnetic poly(glycidyl methacrylate) microspheres containing NH2 groups [P(GMA)-NH2]. They were functionalised with biotynilated anti-Lactobacillus antibody and used for separation of Lactobacillus cells. Preconcentration of cells in a milk product was also tested.

Klíčová slova

Lactobacillus cells; immunomagnetic separation; food products

Klíčová slova v angličtině

Lactobacillus cells; immunomagnetic separation; food products

Autoři

RITTICH, B.; TURKOVÁ, K.; TRACHTOVÁ, Š.; VAŇÁSEK, J.; HORÁK, D.; KOZÁKOVÁ H.; ŠPANOVÁ A.

Rok RIV

2016

Vydáno

24.05.2015

Nakladatel

Institute of Immunology and Experimental Therapy Polish Academy of Science

Místo

Bielawa, Polsko

ISBN

978-83-928488-4-4

Kniha

2nd Polish-Czech Probiotics Conference: Microbiology, Immunology & Allergy,

Strany od

64

Strany do

64

Strany počet

1

BibTex

@misc{BUT114813,
  author="RITTICH, B. and TURKOVÁ, K. and TRACHTOVÁ, Š. and VAŇÁSEK, J. and HORÁK, D. and KOZÁKOVÁ H. and ŠPANOVÁ A.",
  title="Immunomagnetic capture and preconcentration of Lactobacillus cells",
  booktitle="2nd Polish-Czech Probiotics Conference: Microbiology, Immunology & Allergy,",
  year="2015",
  edition="1",
  pages="64--64",
  publisher="Institute of Immunology and Experimental Therapy Polish Academy of Science",
  address="Bielawa, Polsko",
  isbn="978-83-928488-4-4",
  note="Abstract"
}