Detail publikačního výsledku

Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR

Radka Hrudíková, Stanislav Pepeliaev, Vladimír Velebný

Originální název

Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR

Anglický název

Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR

Druh

Abstrakt

Originální abstrakt

Real-time polymerase chain reaction (PCR) has become the preferred method for studying expression. The study of gene expression profiles of whole genome is commonly performed by relying on such technique like cDNA microarray. Real-time PCR technique is commonly used to study expression of tens of genes. Real-time PCR is considered to be the most accurate, because of its high sensitivity and specificity. However to validate obtained data reference gene is needed. We have studied gene expression of bacterial strains of Streptococcus equi subsp. zooepidemicus (SEZ).

Anglický abstrakt

Real-time polymerase chain reaction (PCR) has become the preferred method for studying expression. The study of gene expression profiles of whole genome is commonly performed by relying on such technique like cDNA microarray. Real-time PCR technique is commonly used to study expression of tens of genes. Real-time PCR is considered to be the most accurate, because of its high sensitivity and specificity. However to validate obtained data reference gene is needed. We have studied gene expression of bacterial strains of Streptococcus equi subsp. zooepidemicus (SEZ).

Klíčová slova

Real-Time PCR, reference gene, gene expression stability

Klíčová slova v angličtině

Real-Time PCR, reference gene, gene expression stability

Autoři

Radka Hrudíková, Stanislav Pepeliaev, Vladimír Velebný

Rok RIV

2016

Vydáno

03.12.2015

Nakladatel

Vysoké Učení Technické v Brně, Fakulta chemická, Purkyňova 464/118, Brno 61200

Místo

Brno

ISBN

978-80-214-5290-9

Kniha

Sborník abstraktů

Edice

1

Strany od

65

Strany do

65

Strany počet

1

Plný text v Digitální knihovně

http://hdl.handle.net/

BibTex

@misc{BUT121168,
  author="Radka {Hrudíková}",
  title="Determination of the Reference Genes in Streptococcus equi subsp. zooepidemicus for Real-Time PCR",
  booktitle="Sborník abstraktů",
  year="2015",
  series="1",
  edition="1",
  pages="65--65",
  publisher="Vysoké Učení Technické v Brně, Fakulta chemická, Purkyňova 464/118, Brno 61200",
  address="Brno",
  isbn="978-80-214-5290-9",
  note="Abstract"
}