Detail publikačního výsledku

Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions

ZOVČÁKOVÁ, M.; ŠPANOVÁ, A.; PANTŮČEK, R.; DOŠKAŘ, J.; RITTICH, B.

Originální název

Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions

Anglický název

Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions

Druh

Článek WoS

Originální abstrakt

Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd3+ ions (non-enzymatic cleavage agent) in HEPES buffer (pH 7.5) at 70 °C. Topological forms of plasmids (ccc, oc, lin) produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.

Anglický abstrakt

Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd3+ ions (non-enzymatic cleavage agent) in HEPES buffer (pH 7.5) at 70 °C. Topological forms of plasmids (ccc, oc, lin) produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.

Klíčová slova

lanthanide Nd3+ ions; plasmid DNA; plasmid size; pulsed-field agarose gel electrophoresis; Staphylococcus aureus

Klíčová slova v angličtině

lanthanide Nd3+ ions; plasmid DNA; plasmid size; pulsed-field agarose gel electrophoresis; Staphylococcus aureus

Autoři

ZOVČÁKOVÁ, M.; ŠPANOVÁ, A.; PANTŮČEK, R.; DOŠKAŘ, J.; RITTICH, B.

Rok RIV

2018

Vydáno

26.05.2016

Nakladatel

Elsevier

Místo

USA

ISSN

0003-2697

Periodikum

ANALYTICAL BIOCHEMISTRY

Svazek

507

Číslo

1

Stát

Spojené státy americké

Strany od

66

Strany do

70

Strany počet

5

BibTex

@article{BUT124880,
  author="Monika {Zovčáková} and Alena {Španová} and Roman {Pantůček} and Jiří {Doškař} and Bohuslav {Rittich}",
  title="Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions",
  journal="ANALYTICAL BIOCHEMISTRY",
  year="2016",
  volume="507",
  number="1",
  pages="66--70",
  doi="10.1016/j.ab.2016.05.013",
  issn="0003-2697"
}