Detail publikace

Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres

TRACHTOVÁ, Š. ŠPANOVÁ, A. TÓTH, J. PRETTL, Z. HORÁK, D. GYENIS, J. RITTICH, B.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Dynamic light scattering (DLS) was used to monitor the coil–globule transition of calf thymus and bacterial DNAs by poly(ethylene glycol) (PEG 600 and PEG 6,000) in aqueous NaCl solutions. The contribution of PEG 600 and PEG 6,000 to DNA coil diameter change was investigated. Compaction of DNA molecules was observed for a PEG 6,000 concentration ranging from 5.5 to 8 %. Hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA), poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), and poly(glycidyl methacrylate) (PGMA) microspheres containing carboxyl groups were used for DNA isolation. The highest DNA yield was achieved using 16 % PEG 6,000 and 2.0 M NaCl. The amount of isolated DNA correlated with the content of carboxyl groups on the microsphere surface. RNA adsorption on the surface of the microspheres tested was also studied. RNA recovery was more than one order lower than DNA recovery under the same conditions. All types of microspheres were tested for DNA isolation from crude cell lysates of different dairy products in solutions of 16 % PEG 6,000 and 2.0 M NaCl. Isolated DNAs were without PCR inhibitors, which was demonstrated using real-time PCR including melting analysis of the amplicons.

Klíčová slova

DNA compaction; Magnetic microspheres; DNA isolation; RNA; Polymerase chain reaction (PCR)

Autoři

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; TÓTH, J.; PRETTL, Z.; HORÁK, D.; GYENIS, J.; RITTICH, B.

Rok RIV

2015

Vydáno

1. 4. 2015

Nakladatel

ELSEVIER

Místo

Spojené království Velké Británie a Severního Irska

ISSN

0960-3085

Periodikum

FOOD AND BIOPRODUCTS PROCESSING

Ročník

Číslo

Stát

Spojené království Velké Británie a Severního Irska

Strany od

375

Strany do

381

Strany počet

BibTex

@article{BUT126302,
  author="Štěpánka {Trachtová} and Alena {Španová} and Judit {Tóth} and Zsolt {Prettl} and Daniel {Horák} and János {Gyenis} and Bohuslav {Rittich}",
  title="Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres",
  journal="FOOD AND BIOPRODUCTS PROCESSING",
  year="2015",
  volume="94",
  number="1",
  pages="375--381",
  doi="10.1016/j.fbp.2014.05.001",
  issn="0960-3085"
}