Detail publikačního výsledku

Comparison of flow cytometry and Raman spectroscopy as methods for rapid detremination of intracellular content of polyhydroxyalkanoates in bacterial cells

KUČERA, D.; SAMEK, O.; MÜLLEROVÁ, L.; BENEŠOVÁ, P.; OBRUČA, S.

Originální název

Comparison of flow cytometry and Raman spectroscopy as methods for rapid detremination of intracellular content of polyhydroxyalkanoates in bacterial cells

Anglický název

Comparison of flow cytometry and Raman spectroscopy as methods for rapid detremination of intracellular content of polyhydroxyalkanoates in bacterial cells

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

Polyhydroxyalkanoates (PHAs) are bacterial polyesters which are considered as biodegradable and environmentally-friendly alternative to petrochemical plastics. Biotechnological production of PHAs is complicated by the lack of fast and reliable analytical tool enabling rapid and sensitive determination of PHAs in bacterial cells during the biotechnological process. The most commonly employed method for quantitative analysis of PHAs is gas chromatography, nevertheless, labor intensity and time demands of this methods prevents its application for routine analysis of PHAs during biotechnological process. The aim of this work was to compare two analytical methods which could be used for determination of intracellular content in time-frame of 20 minutes - Flow cytometry and Raman spectroscopy.

Anglický abstrakt

Polyhydroxyalkanoates (PHAs) are bacterial polyesters which are considered as biodegradable and environmentally-friendly alternative to petrochemical plastics. Biotechnological production of PHAs is complicated by the lack of fast and reliable analytical tool enabling rapid and sensitive determination of PHAs in bacterial cells during the biotechnological process. The most commonly employed method for quantitative analysis of PHAs is gas chromatography, nevertheless, labor intensity and time demands of this methods prevents its application for routine analysis of PHAs during biotechnological process. The aim of this work was to compare two analytical methods which could be used for determination of intracellular content in time-frame of 20 minutes - Flow cytometry and Raman spectroscopy.

Klíčová slova

PHAs, Flow cytometry, Raman spectroscopy

Klíčová slova v angličtině

PHAs, Flow cytometry, Raman spectroscopy

Autoři

KUČERA, D.; SAMEK, O.; MÜLLEROVÁ, L.; BENEŠOVÁ, P.; OBRUČA, S.

Vydáno

25.04.2016

ISBN

978-80-86238-91-3

Kniha

4th International Conference on Chemical Technology ICCT 2016 Sborník příspěvků

Strany od

488

Strany do

492

Strany počet

5

URL

https://dev8-admin.morbo.puxdesign.cz/Amca-ICCT/media/content/2016/Proceedings_ICCT2016.pdf

Plný text v Digitální knihovně

http://hdl.handle.net/

BibTex

@inproceedings{BUT130036,
  author="Dan {Kučera} and Ota {Samek} and Lucie {Müllerová} and Pavla {Benešová} and Stanislav {Obruča}",
  title="Comparison of flow cytometry and Raman spectroscopy as methods for rapid detremination of intracellular content of polyhydroxyalkanoates in bacterial cells",
  booktitle="4th International Conference on Chemical Technology ICCT 2016 Sborník příspěvků",
  year="2016",
  pages="488--492",
  isbn="978-80-86238-91-3",
  url="https://dev8-admin.morbo.puxdesign.cz/Amca-ICCT/media/content/2016/Proceedings_ICCT2016.pdf"
}