Detail publikačního výsledku

Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study

MÁROVÁ, I.; SZOTKOWSKI, M.; VANĚK, M.; RAPTA, M.; BYRTUSOVÁ, D.; MIKHEICHYK, N.; HÁRONIKOVÁ, A.

Originální název

Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study

Anglický název

Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study

Druh

Článek WoS

Originální abstrakt

Red yeast strains are ubiquitous microorganisms which accumulate substantial amounts of lipids and lipid-soluble metabolites. Red yeasts utilize many waste substrates of different origin. In this work red yeasts strains ( Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. As a carbon source waste animal fat and its hydrolysis products were used. Hydrolysis of animal fat was tested in alkaline as well as acidic conditions. As the substrate glucose (control), glycerol, crude animal fat, acid fat hydrolyzate and hydrolysate: glucose 1:2 were used. Screening of growth and metabolic activity of red yeasts was performed by flow cytometry. Extracellular lipase production was monitored as adaptation mechanism. Carotenoids, ergosterol and ubiquinone were quantified by HPLC/PDA/MS/ESI and the biomass was evaluated gravimetricaly.

Anglický abstrakt

Red yeast strains are ubiquitous microorganisms which accumulate substantial amounts of lipids and lipid-soluble metabolites. Red yeasts utilize many waste substrates of different origin. In this work red yeasts strains ( Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. As a carbon source waste animal fat and its hydrolysis products were used. Hydrolysis of animal fat was tested in alkaline as well as acidic conditions. As the substrate glucose (control), glycerol, crude animal fat, acid fat hydrolyzate and hydrolysate: glucose 1:2 were used. Screening of growth and metabolic activity of red yeasts was performed by flow cytometry. Extracellular lipase production was monitored as adaptation mechanism. Carotenoids, ergosterol and ubiquinone were quantified by HPLC/PDA/MS/ESI and the biomass was evaluated gravimetricaly.

Klíčová slova

carotenogenic yeasts, lipids, animal waste

Klíčová slova v angličtině

carotenogenic yeasts, lipids, animal waste

Autoři

MÁROVÁ, I.; SZOTKOWSKI, M.; VANĚK, M.; RAPTA, M.; BYRTUSOVÁ, D.; MIKHEICHYK, N.; HÁRONIKOVÁ, A.

Rok RIV

2018

Vydáno

17.10.2017

Nakladatel

European Biotechnology Thematic Network Association

Místo

Editorial office, Piazza Università 1, Perugia, Italy

ISSN

2564-615X

Periodikum

The EuroBiotech Journal

Svazek

1

Číslo

4

Stát

Italská republika

Strany od

310

Strany do

318

Strany počet

9

URL

http://eurobiotechjournal.org/sayilar/93/buyuk/08_Res_Art_Marova.pdf

Plný text v Digitální knihovně

http://hdl.handle.net/

BibTex

@article{BUT143410,
  author="Ivana {Márová} and Martin {Szotkowski} and Martin {Vaněk} and Marek {Rapta} and Dana {Byrtusová} and Nadzeya {Mikheichyk} and Andrea {Němcová}",
  title="Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study",
  journal="The EuroBiotech Journal",
  year="2017",
  volume="1",
  number="4",
  pages="310--318",
  doi="10.24190/ISSN2564-615X/2017/04.08",
  issn="2564-615X",
  url="http://eurobiotechjournal.org/sayilar/93/buyuk/08_Res_Art_Marova.pdf"
}

Dokumenty

[2564615X - The EuroBiotech Journal] Utilization of animal fat waste as carbon source by carotenogenic yeasts – a screening study