Detail publikačního výsledku
High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group
KONEČNÁ, J.; CHVALKOVSKÁ, E.; KNÁPKOVÁ, M.; ŠPANOVÁ, A.; RITTICH, B.; BRÁZDA, V.
Originální název
High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group
Anglický název
High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group
Druh
Stať ve sborníku v databázi WoS či Scopus
Originální abstrakt
Molecular methods based on the amplification of 16S ribosomal RNA gene analysis are more rapid, reliable, and reproducible and have greater sensitivity. High resolution melting (HRM) curve analysis is a simple, low-cost, closed tube method for amplicon discrimination and easy connection with real-time polymerase chain reaction (PCR). In this contribution, we report rapid species identification of strains belonging to the Lactobacillus casei group using HRM-PCR. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Two sets of targeted gene fragments primers (groEL, BSH) were tested for amplification of the 16S rRNA gene. Use of groEL primers pair successfully identify strains belong to the Lactobacillus casei group. The variance between used extraction methods for evidence of HRM curves was found.
Anglický abstrakt
Molecular methods based on the amplification of 16S ribosomal RNA gene analysis are more rapid, reliable, and reproducible and have greater sensitivity. High resolution melting (HRM) curve analysis is a simple, low-cost, closed tube method for amplicon discrimination and easy connection with real-time polymerase chain reaction (PCR). In this contribution, we report rapid species identification of strains belonging to the Lactobacillus casei group using HRM-PCR. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Two sets of targeted gene fragments primers (groEL, BSH) were tested for amplification of the 16S rRNA gene. Use of groEL primers pair successfully identify strains belong to the Lactobacillus casei group. The variance between used extraction methods for evidence of HRM curves was found.
Klíčová slova
Lactobacillus casei; DNA isolation; High-Resolution Melting Analysis
Klíčová slova v angličtině
Lactobacillus casei; DNA isolation; High-Resolution Melting Analysis
Autoři
KONEČNÁ, J.; CHVALKOVSKÁ, E.; KNÁPKOVÁ, M.; ŠPANOVÁ, A.; RITTICH, B.; BRÁZDA, V.
Rok RIV
2019
Vydáno
25.04.2018
ISBN
978-80-223-4517-0
Kniha
Študentská vedecká konferencia PriF UK 2018 Zborník recenzovaných príspevkov
Strany od
302
Strany do
307
Strany počet
1652
BibTex
@inproceedings{BUT147532,
author="Jana {Konečná} and Eva {Chvalkovská} and Monika {Knápková} and Alena {Španová} and Bohuslav {Rittich} and Václav {Brázda}",
title="High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group",
booktitle="Študentská vedecká konferencia PriF UK 2018 Zborník recenzovaných príspevkov",
year="2018",
number="první",
pages="302--307",
isbn="978-80-223-4517-0"
}