Detail publikačního výsledku

Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles

KONEČNÁ, J.; ROMANOVSKÁ, D.; TRACHTOVÁ, Š.

Originální název

Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles

Anglický název

Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles

Druh

Článek WoS

Originální abstrakt

Isolation of deoxyribonucleic acid is an important step in the molecular diagnostics of microorganisms. A high quality of isolated deoxyribonucleic acid is necessary for deoxyribonucleic acid amplification by the polymerase chain reaction. The conventional deoxyribinucleic acid isolation using phenol-chloroform extraction and deoxyribonucleic acid precipitation in ethanol is time-consumingand requires the use of oxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The ain of this work was to use two different types of deoxyribonucleic acid in separation mixtures were measured using ultraviolet spectrophotometry.

Anglický abstrakt

Isolation of deoxyribonucleic acid is an important step in the molecular diagnostics of microorganisms. A high quality of isolated deoxyribonucleic acid is necessary for deoxyribonucleic acid amplification by the polymerase chain reaction. The conventional deoxyribinucleic acid isolation using phenol-chloroform extraction and deoxyribonucleic acid precipitation in ethanol is time-consumingand requires the use of oxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The ain of this work was to use two different types of deoxyribonucleic acid in separation mixtures were measured using ultraviolet spectrophotometry.

Klíčová slova

Magnetic particles, Solid-phase extraction, Polymerase chain reaction, Deoxyribonucleic acid isolation

Klíčová slova v angličtině

Magnetic particles, Solid-phase extraction, Polymerase chain reaction, Deoxyribonucleic acid isolation

Autoři

KONEČNÁ, J.; ROMANOVSKÁ, D.; TRACHTOVÁ, Š.

Rok RIV

2019

Vydáno

01.05.2019

Nakladatel

Chemical papers

Místo

Institute of Chemistry, Slovak Academy of Sciences

ISSN

1336-9075

Periodikum

Chemical Papers

Svazek

120

Číslo

13

Stát

Slovenská republika

Strany od

1

Strany do

9

Strany počet

9

URL

https://link.springer.com/article/10.1007/s11696-018-00675-9

BibTex

@article{BUT151274,
  author="Jana {Konečná} and Denisa {Langová} and Štěpánka {Trachtová}",
  title="Optimalization of deoxyribonucleic acid extraction using variuos types of magnetic particles",
  journal="Chemical Papers",
  year="2019",
  volume="120",
  number="13",
  pages="1--9",
  doi="10.1007/s11696-018-00675-9",
  issn="1336-9075",
  url="https://link.springer.com/article/10.1007/s11696-018-00675-9"
}