Detail publikačního výsledku
Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth
VANĚK, M.; MRAVEC, F.; SZOTKOWSKI, M.; BYRTUSOVÁ, D.; HÁRONIKOVÁ, A.; MÁROVÁ, I.
Originální název
Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth
Anglický název
Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth
Druh
Článek WoS
Originální abstrakt
Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in compartments of yeast cell, especially cytoplasmic membrane.
Anglický abstrakt
Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in compartments of yeast cell, especially cytoplasmic membrane.
Klíčová slova
autofluorescence, carotenoids, Cystofilobasidium capitatum, fluorescence lifetime imaging, red yeasts
Klíčová slova v angličtině
autofluorescence, carotenoids, Cystofilobasidium capitatum, fluorescence lifetime imaging, red yeasts
Autoři
VANĚK, M.; MRAVEC, F.; SZOTKOWSKI, M.; BYRTUSOVÁ, D.; HÁRONIKOVÁ, A.; MÁROVÁ, I.
Rok RIV
2019
Vydáno
10.04.2018
Nakladatel
De Gruyter Open
Místo
Itálie
ISSN
2564-615X
Periodikum
EuroBiotech Journal
Svazek
2
Číslo
2
Stát
Italská republika
Strany od
114
Strany do
120
Strany počet
7
URL
BibTex
@article{BUT155466,
author="Martin {Vaněk} and Filip {Mravec} and Martin {Szotkowski} and Dana {Byrtusová} and Andrea {Němcová} and Ivana {Márová}",
title="Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth",
journal="EuroBiotech Journal",
year="2018",
volume="2",
number="2",
pages="114--120",
doi="10.2478/ebtj-2018-0015",
url="http://www.degruyter.com/view/j/ebtj.2018.2.issue-2/ebtj-2018-0015/ebtj-2018-0015.xml?format=INT"
}Dokumenty