Detail publikace

Příprava nanočástic na bázi PHB-liposomy a studium jejich toxicity in vitro

BOKROVÁ, J. MÁROVÁ, I. MATOUŠKOVÁ, P. PAVELKOVÁ, R.

Originální název

Fabrication of novel PHB-liposome nanoparticles and study of their toxicity in vitro

Český název

Příprava nanočástic na bázi PHB-liposomy a studium jejich toxicity in vitro

Anglický název

Fabrication of novel PHB-liposome nanoparticles and study of their toxicity in vitro

Typ

článek v časopise

Jazyk

en

Originální abstrakt

n this work, novel combined PHB-liposome nanoparticles were synthesized and characterized, and their biocompatability with mammalian cells was assessed in vitro. Incorporation of PHB into liposome particles led to preservation of small size (123.8 ± 0.9 nm; evaluated by DLS) and colloidal stability of particles for 2 months in water environment. Cytotoxicity studies using MTT test with two different skin keratinocytes cell lines (HEK and HaCaT) showed significant dose-dependent differences in cell viability after exposure to liposome and PHB liposome particles in vitro.

Český abstrakt

V práci byly připraveny a charakterizovány nanočástice na bázi PHB-liposomy. Jejich biokompatibilta byla studována s využitím savčích buněčných kultur in vitro. Byla stanovena velikost (123.8 ± 0.9 nm; DLS) a koloidní stabilita částic po dobu 2 měsíců ve vodném prostředí.. Cytotoxicita byla studována pomocí MTT testu na keratinocytechs (HEK; HaCaT).

Anglický abstrakt

n this work, novel combined PHB-liposome nanoparticles were synthesized and characterized, and their biocompatability with mammalian cells was assessed in vitro. Incorporation of PHB into liposome particles led to preservation of small size (123.8 ± 0.9 nm; evaluated by DLS) and colloidal stability of particles for 2 months in water environment. Cytotoxicity studies using MTT test with two different skin keratinocytes cell lines (HEK and HaCaT) showed significant dose-dependent differences in cell viability after exposure to liposome and PHB liposome particles in vitro.

Klíčová slova

nančástice, PHB-liposomy, cytotoxicita

Vydáno

11.02.2019

Nakladatel

Springer Verlag, SRN

Místo

SRN

Strany od

3

Strany do

11

Strany počet

8

BibTex


@article{BUT155931,
  author="Jitka {Bokrová} and Ivana {Márová} and Petra {Matoušková} and Renata {Pavelková}",
  title="Fabrication of novel PHB-liposome nanoparticles and study of their toxicity in vitro",
  annote="n this work, novel combined PHB-liposome nanoparticles were synthesized and characterized, and their biocompatability with mammalian cells was assessed in vitro. Incorporation of PHB into liposome particles led to preservation of small size (123.8 ± 0.9 nm; evaluated by DLS) and colloidal stability of particles for 2 months in water environment. Cytotoxicity studies using MTT test with two different skin keratinocytes cell lines (HEK and HaCaT) showed significant dose-dependent differences in cell viability after exposure to liposome and PHB liposome particles in vitro.",
  address="Springer Verlag, SRN",
  chapter="155931",
  doi="10.1007/s11051-019-4484-7",
  howpublished="print",
  institution="Springer Verlag, SRN",
  number="3",
  volume="2",
  year="2019",
  month="february",
  pages="3--11",
  publisher="Springer Verlag, SRN",
  type="journal article"
}

Odpovědnost: Ing. Jan Brada