Detail publikace

Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery

MILOSAVLJEVIĆ, V. JAMROZ, E. GAGIĆ, M. HADDAD, Y. MICHÁLKOVÁ, H. BÁLKOVÁ, R. TESAŘOVÁ, B. MOULICK, A. HEGER, Z. RICHTERA, L. KOPEL, P. ADAM, V.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Minimization of drug side effects is a hallmark of advanced targeted therapy. Herein we describe the synthesis of polysaccharide-based nanocapsules prepared from furcellaran and chitosan via layer-by-layer deposition using electrostatic interaction. Using doxorubicin as a model drug, prepared nanocapsules showed excellent drug loading properties and release influence by pH and stability. Targeted delivery of doxorubicin was achieved by nanocapsule surface modification using homing peptide (seq SMSIARLC). The synthesized nanocapsules possess excellent compatibility to eukaryotic organisms. In the case of nonmalignant cells (PNT1A and HEK-293), toxicity tests revealed the absences of DNA fragmentation, apoptosis, necrosis, and also disruption of erythrocyte membranes. In contrast, results from treatment of malignant cell lines (MDA-MB-231 and PC3) indicate good anticancer effects of synthesized bionanomaterial. Internalization studies revealed the nanocapsule's ability to enter the malignant cell lines by endocytosis and triggering the apoptosis. The occurrence of apoptosis is mostly connected to the presence of ROS and inability of DNA damage reparation. Additionally, the obtained results strongly indicate that peptide modification increases the speed of nanocapsule internalization into malignant cell lines while simultaneously nonmalignant cell lines are untouched by nanocapsules highlighting the strong selectivity of the peptide.

Klíčová slova

Nanocapsules, Furcellaran, Chitosan, Peptide, Drug, Selective Delivery

Autoři

MILOSAVLJEVIĆ, V.; JAMROZ, E.; GAGIĆ, M.; HADDAD, Y.; MICHÁLKOVÁ, H.; BÁLKOVÁ, R.; TESAŘOVÁ, B.; MOULICK, A.; HEGER, Z.; RICHTERA, L.; KOPEL, P.; ADAM, V.

Vydáno

1. 2. 2020

ISSN

1525-7797

Periodikum

BIOMACROMOLECULES

Ročník

Číslo

Stát

Spojené státy americké

Strany od

Strany do

Strany počet

URL

https://www.scopus.com/record/display.uri?eid=2-s2.0-85076242491&origin=resultslist&sort=plf-f&src=s&st1=balkova+r&st2=&sid=76738c59a7fc3ce72fb67fbe36548196&sot=b&sdt=b&sl=22&s=AUTHOR-NAME%28balkova+r%29&relpos=3&citeCnt=0&searchTerm=

BibTex

@article{BUT161729,
  author="Vedran {Milosavljević} and Ewelina {Jamroz} and Milica {Gagić} and Yazan Abdulmajeed Eyadh {Haddad} and Hana {Michálková} and Radka {Bálková} and Barbora {Tesařová} and Amitava {Moulick} and Zbyněk {Heger} and Lukáš {Richtera} and Pavel {Kopel} and Vojtěch {Adam}",
  title="Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery",
  journal="BIOMACROMOLECULES",
  year="2020",
  volume="21",
  number="2",
  pages="1--17",
  doi="10.1021/acs.biomac.9b01175",
  issn="1525-7797",
  url="https://www.scopus.com/record/display.uri?eid=2-s2.0-85076242491&origin=resultslist&sort=plf-f&src=s&st1=balkova+r&st2=&sid=76738c59a7fc3ce72fb67fbe36548196&sot=b&sdt=b&sl=22&s=AUTHOR-NAME%28balkova+r%29&relpos=3&citeCnt=0&searchTerm="
}