Detail publikace

G-quadruplexes in the Archaea Domain

BRÁZDA, V. LUO, Y. BARTAS, M. KAURA, P. PORUBIAKOVÁ, O. ŠŤASTNÝ, J. PEČINKA, P. VERGA, D. DA CUNHA, V. S. TAKAHASHI, T. FORTERRE, P. MYLLYKALLIO, H. FOJTA, M. MERGNY, J.

Originální název

G-quadruplexes in the Archaea Domain

Anglický název

G-quadruplexes in the Archaea Domain

Jazyk

en

Originální abstrakt

The importance of unusual DNA structures in the regulation of basic cellular processes is an emerging field of research. Amongst local non-B DNA structures, G-quadruplexes (G4s) have gained in popularity during the last decade, and their presence and functional relevance at the DNA and RNA level has been demonstrated in a number of viral, bacterial and eukaryotic genomes, including humans. Here, we performed the first systematic search of G4-forming sequences in all archaeal genomes available in the NCBI database. We investigated the presence and locations of G-quadruplex forming sequences using the G4Hunter algorithm. G-quadruplex prone sequences were identified in all archaeal species, with highly significant differences in frequency, from 0.037 to 15.31 potential quadruplex sequences per kb. While G4 forming sequences were extremely abundant in Hadesarchaea archeon (strikingly, more than 50% of the Hadesarchaea archaeon isolate WYZ-LMO6 genome is potential part of a G4-motif), they were very rare in the Parvarchaeota phylum. The presence of G-quadruplex forming sequences does not follow a random distribution with an over-representation in non-coding RNA, suggesting possible roles for ncRNA regulation. These data illustrate the unique and non-random localization of G-quadruplexes in Archaea.

Anglický abstrakt

The importance of unusual DNA structures in the regulation of basic cellular processes is an emerging field of research. Amongst local non-B DNA structures, G-quadruplexes (G4s) have gained in popularity during the last decade, and their presence and functional relevance at the DNA and RNA level has been demonstrated in a number of viral, bacterial and eukaryotic genomes, including humans. Here, we performed the first systematic search of G4-forming sequences in all archaeal genomes available in the NCBI database. We investigated the presence and locations of G-quadruplex forming sequences using the G4Hunter algorithm. G-quadruplex prone sequences were identified in all archaeal species, with highly significant differences in frequency, from 0.037 to 15.31 potential quadruplex sequences per kb. While G4 forming sequences were extremely abundant in Hadesarchaea archeon (strikingly, more than 50% of the Hadesarchaea archaeon isolate WYZ-LMO6 genome is potential part of a G4-motif), they were very rare in the Parvarchaeota phylum. The presence of G-quadruplex forming sequences does not follow a random distribution with an over-representation in non-coding RNA, suggesting possible roles for ncRNA regulation. These data illustrate the unique and non-random localization of G-quadruplexes in Archaea.

Plný text v Digitální knihovně

Dokumenty

BibTex


@article{BUT165313,
  author="Václav {Brázda} and Yu {Luo} and Martin {Bartas} and Patrik {Kaura} and Otília {Porubiaková} and Jiří {Šťastný} and Petr {Pečinka} and Daniela {Verga} and Violette {Da Cunha} and Tomio {S. Takahashi} and Patrick {Forterre} and Hannu {Myllykallio} and Miroslav {Fojta} and Jean-Louis {Mergny}",
  title="G-quadruplexes in the Archaea Domain",
  annote="The importance of unusual DNA structures in the regulation of basic cellular processes is an emerging field of research. Amongst local non-B DNA structures, G-quadruplexes (G4s) have gained in popularity during the last decade, and their presence and functional relevance at the DNA and RNA level has been demonstrated in a number of viral, bacterial and eukaryotic genomes, including humans. Here, we performed the first systematic search of G4-forming sequences in all archaeal genomes available in the NCBI database. We investigated the presence and locations of G-quadruplex forming sequences using the G4Hunter algorithm. G-quadruplex prone sequences were identified in all archaeal species, with highly significant differences in frequency, from 0.037 to 15.31 potential quadruplex sequences per kb. While G4 forming sequences were extremely abundant in Hadesarchaea archeon (strikingly, more than 50% of the Hadesarchaea archaeon isolate WYZ-LMO6 genome is potential part of a G4-motif), they were very rare in the Parvarchaeota phylum. The presence of G-quadruplex forming sequences does not follow a random distribution with an over-representation in non-coding RNA, suggesting possible roles for ncRNA regulation. These data illustrate the unique and non-random localization of G-quadruplexes in Archaea.",
  address="MDPI",
  chapter="165313",
  doi="10.3390/biom10091349",
  howpublished="online",
  institution="MDPI",
  number="9",
  volume="10",
  year="2020",
  month="september",
  pages="1--23",
  publisher="MDPI",
  type="journal article in Web of Science"
}