Detail publikace

Evolutionary engineering approach for adaptation of PHA producing strain Halomonas halophila to levulinic and acetic acid

NOVÁČKOVÁ, I. CHATRNÁ, V. SLANINOVÁ, E. POŘÍZKA, J. SEDLÁČEK, P. OBRUČA, S.

Originální název

Evolutionary engineering approach for adaptation of PHA producing strain Halomonas halophila to levulinic and acetic acid

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Evolutionary engineering provides approaches useful for obtaining of microorganisms with desired characteristics on phenotype level (e.g. more effective growth, ability of utilization of different carbon sources, etc.). Characteristics of polyhydroxyalkanoates (PHAs), microbial biodegradable and biocompatible polymers, strongly depend on monomer composition. These materials could be advantageously used as an alternative of traditional petrochemical plastics. Evolved microbial strains obtained during evolutionary engineering experiments could be used for production of PHA with required properties for selected purposes. Promising halophilic PHA producer Halomonas halophila (CCM 3662) was exposed to microbial inhibitors commonly presented in hydrolysates of lignocellulosic biomass, namely acetic and levulinic acid. Multiple serial transfers of cell cultures in Erlenmeyer flasks after 48 hours were provided within adaptation experiments for more than 80 passages. Every passage has been basically characterized and selected preserved ones were also compared with wild-type strain considering potential of PHAs accumulation, effectivity of utilization of organic acids, testing of robustness and others features.

Klíčová slova

evolutionary engineering, polyhydroxyalkanoates, Halomonas halophila, levulinic acid, acidic acid

Autoři

NOVÁČKOVÁ, I.; CHATRNÁ, V.; SLANINOVÁ, E.; POŘÍZKA, J.; SEDLÁČEK, P.; OBRUČA, S.

Vydáno

25. 2. 2021

ISSN

1314-3530

Periodikum

Biotechnology and Biotechnological Equipment

Ročník

35

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

25

Strany do

25

Strany počet

1

BibTex

@misc{BUT169835,
  author="Ivana {Nováčková} and Vendula {Hrabalová} and Eva {Slaninová} and Jaromír {Pořízka} and Petr {Sedláček} and Stanislav {Obruča}",
  title="Evolutionary engineering approach for adaptation of PHA producing strain Halomonas halophila to levulinic and acetic acid",
  year="2021",
  journal="Biotechnology and Biotechnological Equipment",
  volume="35",
  number="1",
  pages="25--25",
  issn="1314-3530",
  note="abstract"
}