Detail publikačního výsledku

Newly designed primers targeting limonene synthase

ROMANOVSKÁ, D.; FIALOVÁ, L.; MÁROVÁ, I.

Originální název

Newly designed primers targeting limonene synthase

Anglický název

Newly designed primers targeting limonene synthase

Druh

Abstrakt

Originální abstrakt

Cosmetic products with ingredients of plant origin promise beneficial effects on the customer health. Those may contain allergens that belong to the group of 24 fragrance allergens that can negatively affect the health of sensitive individuals. One of these allergens is d-limonene present for example in citrus fruits. The presence of plant DNA in the cosmetic product can be detected using techniques of molecular biotechnology, even if it is present in very small quantity. New primers Lim2 F/R, targeting limonene synthase, were designed to confirm the presence of the citrus DNA in a leave-on cosmetic product. The DNA of Citrus limon was isolated from the leave-on cosmetic product and the specific sequence was amplified using real-time PCR. The presence of amplicons of specific size was confirmed using agarose electrophoresis.

Anglický abstrakt

Cosmetic products with ingredients of plant origin promise beneficial effects on the customer health. Those may contain allergens that belong to the group of 24 fragrance allergens that can negatively affect the health of sensitive individuals. One of these allergens is d-limonene present for example in citrus fruits. The presence of plant DNA in the cosmetic product can be detected using techniques of molecular biotechnology, even if it is present in very small quantity. New primers Lim2 F/R, targeting limonene synthase, were designed to confirm the presence of the citrus DNA in a leave-on cosmetic product. The DNA of Citrus limon was isolated from the leave-on cosmetic product and the specific sequence was amplified using real-time PCR. The presence of amplicons of specific size was confirmed using agarose electrophoresis.

Klíčová slova

Cosmetics, plant DNA, Citrus limon, primers, PCR

Klíčová slova v angličtině

Cosmetics, plant DNA, Citrus limon, primers, PCR

Autoři

ROMANOVSKÁ, D.; FIALOVÁ, L.; MÁROVÁ, I.

Rok RIV

2021

Vydáno

26.02.2021

Nakladatel

Taylor and Francis

Místo

Prague

ISBN

1310-2818

Kniha

Biotechnology & Biotechnological Equipment, Special Issue:European Biotechnology Congress 2020

Edice

35

ISSN

1314-3530

Periodikum

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

Svazek

35

Číslo

Sup 1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

119

Strany do

119

Strany počet

1

URL

https://www.tandfonline.com/doi/full/10.1080/13102818.2020.1871545?fbclid=IwAR0gLYRgommM-eySZC1qddUErA0VWyAlsj5S0gYm3QG8A0HLbrOJyJyu624

BibTex

@misc{BUT170131,
  author="Denisa {Langová} and Lenka {Fialová} and Ivana {Márová}",
  title="Newly designed primers targeting limonene synthase",
  booktitle="Biotechnology & Biotechnological Equipment, Special Issue:European Biotechnology Congress 2020",
  year="2021",
  series="35",
  edition="sup1",
  journal="BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT",
  volume="35",
  number="Sup 1",
  pages="119--119",
  publisher="Taylor and Francis",
  address="Prague",
  isbn="1310-2818",
  issn="1310-2818",
  url="https://www.tandfonline.com/doi/full/10.1080/13102818.2020.1871545?fbclid=IwAR0gLYRgommM-eySZC1qddUErA0VWyAlsj5S0gYm3QG8A0HLbrOJyJyu624",
  note="Abstract"
}