Detail publikačního výsledku

Extremophiles – Platform strains for sustainable production of polyhydroxyalkanoates

PERNICOVÁ, I.; KUČERA, D.; NOVÁČKOVÁ, I.; VODIČKA, J.; KOVALČÍK, A.; OBRUČA, S.

Originální název

Extremophiles – Platform strains for sustainable production of polyhydroxyalkanoates

Anglický název

Extremophiles – Platform strains for sustainable production of polyhydroxyalkanoates

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

Polyhydroxyalkanoates (PHA) are biodegradable polyesters, which are produced by various bacteria including numerous halophiles. Employment of halophilic strain for PHA production brings numerous benefits such as robustness of the process against contamination by ubiquitous mesophiles or possibility to isolate polymer from bacterial biomass via hypotonic lysis. In this work, we screened three moderate halophiles – Halomonas halophila, Halomonas organivorans and Halomonas salina for the presence of phaC gene encoding for PHA synthase and, subsequently, we have investigated their PHA production potential on various sugars. Among tested strains, H. organivorans demonstrated extraordinary capacity of PHA production in particular on galactose and mannose since on these saccharides PHA content in dried bacterial cells reached 83 and 90 wt. % on mannose and galactose, respectively.

Anglický abstrakt

Polyhydroxyalkanoates (PHA) are biodegradable polyesters, which are produced by various bacteria including numerous halophiles. Employment of halophilic strain for PHA production brings numerous benefits such as robustness of the process against contamination by ubiquitous mesophiles or possibility to isolate polymer from bacterial biomass via hypotonic lysis. In this work, we screened three moderate halophiles – Halomonas halophila, Halomonas organivorans and Halomonas salina for the presence of phaC gene encoding for PHA synthase and, subsequently, we have investigated their PHA production potential on various sugars. Among tested strains, H. organivorans demonstrated extraordinary capacity of PHA production in particular on galactose and mannose since on these saccharides PHA content in dried bacterial cells reached 83 and 90 wt. % on mannose and galactose, respectively.

Klíčová slova

polyhydroxyalakanoates, halophiles, spent coffe grounds

Klíčová slova v angličtině

polyhydroxyalakanoates, halophiles, spent coffe grounds

Autoři

PERNICOVÁ, I.; KUČERA, D.; NOVÁČKOVÁ, I.; VODIČKA, J.; KOVALČÍK, A.; OBRUČA, S.

Rok RIV

2022

Vydáno

27.05.2019

Nakladatel

Trans Tech Publications Ltd

ISBN

9783035714449

Kniha

Materials Science Forum

Strany od

74

Strany do

79

Strany počet

6

BibTex

@inproceedings{BUT176969,
  author="Iva {Buchtíková} and Dan {Kučera} and Ivana {Nováčková} and Juraj {Vodička} and Adriána {Kovalčík} and Stanislav {Obruča}",
  title="Extremophiles – Platform strains for sustainable production of polyhydroxyalkanoates",
  booktitle="Materials Science Forum",
  year="2019",
  number="955",
  pages="74--79",
  publisher="Trans Tech Publications Ltd",
  doi="10.4028/www.scientific.net/MSF.955.74",
  isbn="9783035714449"
}