PHA co-polymers production in two cyanobacterial strains from Synechocystis family

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Cyanobacteria are ecologically extremely important phototrophic gram-negative which belong to the prokaryotes, cyanobacteria contains plastids so they capable to do oxygenic photosynthesis. Cyanobacteria synthetize many interesting metabolites such as glycogen, lipids, carotenoids, but the most interesting metabolites are polyhydroxyalkanoates (PHA). PHA are biodegradable biopolymers with similar properties as petrochemical plastics and they occure in bacteria in granules. Numerous prokaryotes including cyanobacteria use PHA as a storage of carbon and energy in form of intracellular granules. The most common type of PHA is poly-3-hydroxybutyrate (PHB). PHA can be produced by heterotrophic bacteria as well, such as Cupriavidus necator H16 or Methylobacterium rhodesianum. PHB raises fitness of bacteria because it helps the bacteria to survive the stress conditions – osmotic stress, UV radiation, freezing or high temperatures. Both strains are capable of co-polymer synthesis in presence of its structural precursor. The composition of co-polymer differs in Synechocystis sp. PCC 6803 and Synechocystis salina CCALA 192 and the composition of co-polymers also varies depending on the precursor concentration. Synechocystis sp. PCC 6803 and CCALA 192 cultivated with acetate and γ-butyrolakton accumulated co-polymer of 3-hydroxybutyrate (3HB) and 4-hydroxybutyrate (4HB). 4HB was for the first time in cyanobacteria reported in Synechococcus sp. PCC 7002 in 2015 an in PCC 6803 in 2020. The total amount of PHA as well as yield of biomass was lower than in a control due to the toxic properties of γ-butyrolakton.

Cyanobacteria, co-polymer, Synechocystis

ŠEDRLOVÁ, Z.; SLANINOVÁ, E.; OBRUČA, S.

28. 4. 2022

MASARYK UNIVERSITY PRESS

Brno

978-80-280-0040-0

The Biomania Student Scientific Meeting

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